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LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin
http://hdl.handle.net/10076/00017114
http://hdl.handle.net/10076/00017114d1b44b08-17ea-4a6d-b0d3-8124ac0fb67a
名前 / ファイル | ライセンス | アクション |
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2016DM021 (1.3 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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公開日 | 2017-11-07 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Integrin | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Thrombomodulin | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Inflammation | |||||||||||
キーワード | ||||||||||||
言語 | en | |||||||||||
主題Scheme | Other | |||||||||||
主題 | Adhesion molecules | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
資源タイプ | doctoral thesis | |||||||||||
アクセス権 | ||||||||||||
アクセス権 | open access | |||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
著者 |
川本, 英嗣
× 川本, 英嗣
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | LFA-1 (aLb2) and Mac-1 (aMb2) integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells. Here we have shown that the anticoagulant molecule thrombomodulin (TM), found on the surface of endothelial cells, functions as a potentially new ligand for leukocyte integrins. We generated a recombinant extracellular domain of human TM and Fc fusion protein (TM-domains 123-Fc), and showed that pheripheral blood mononuclear cells (PBMCs) bind to TM-domains 123-Fc dependent upon integrin activation. We then demonstrated that aL integrin-blocking mAb, aM integrin-blocking mAb, and b2 integrin-blocking mAb inhibited the binding of PBMCs to TM-domains 123-Fc. Furthermore, we show that the serine/threonine-rich domain (domain 3) of TM is required for the interaction with the LFA-1 (aLb2) and Mac-1 (aMb2) integrins to occur on PBMCs. These results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to TM, thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. In fact, integrin-TM interactions might be involved in the dynamic regulation of leukocyte adhesion with endothelial cells. | |||||||||||
内容記述 | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | 本文 / Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine; Emergency and Critical Care Center, Mie University Hospital | |||||||||||
内容記述 | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | 8p | |||||||||||
書誌情報 |
発行日 2017-03-24 |
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DOI | ||||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.1016/j.bbrc.2016.04.007 | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
出版者 | ||||||||||||
出版者 | 三重大学 | |||||||||||
出版者(ヨミ) | ||||||||||||
ミエダイガク | ||||||||||||
学位名 | ||||||||||||
学位名 | 博士(医学) | |||||||||||
学位授与機関 | ||||||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||||
学位授与機関識別子 | 14101 | |||||||||||
学位授与機関名 | 三重大学 | |||||||||||
学位授与年月日 | ||||||||||||
学位授与年月日 | 2017-03-24 | |||||||||||
学位授与番号 | ||||||||||||
学位授与番号 | 甲医学第1814号 | |||||||||||
ノート | ||||||||||||
Biochemical and Biophysical Research Communications, 473(4), 2016, pp. 1005-1012, ISSN:0006-291X に掲載 | ||||||||||||
資源タイプ(三重大) | ||||||||||||
Doctoral Dissertation / 博士論文 |