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ScFv化抗テネイシンC抗体による心疾患の分子標的イメージング診断法の確立
http://hdl.handle.net/10076/15501
http://hdl.handle.net/10076/15501c0d9e14a-530e-4883-8077-553fd5c5a620
名前 / ファイル | ライセンス | アクション |
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30K17918.pdf (306.2 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2017-01-13 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | ScFv化抗テネイシンC抗体による心疾患の分子標的イメージング診断法の確立 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ScFv型抗体 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | テネイシン-C | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 分子イメージング | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 虚血性心疾患 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
著者 |
下條, 尚志
× 下條, 尚志 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | テネイシン-C(TN-C)は虚血性心疾患などの炎症部位に一過性に高発現する。TN-Cを特異的に認識する抗体を用いて、分子診断薬として改良可能かどうかを検証した。ハイブリドーマのRNAから抗TN-C抗体のH鎖可変領域(VH)とL鎖可変領域(VL)遺伝子をクローニングし、重複伸長PCRを行い、VH-linker-VL遺伝子(scFv遺伝子)を大腸菌、in vitroセルフリー(RTS小麦胚芽)の発現系、哺乳類細胞株(HEK293)へそれぞれ遺伝子導入した。その結果、HEK293において最も多く発現量が得られた。準備が整い次第、心筋炎モデル動物に注入して病巣への集積を確認する予定である。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tenascin-C (TN-C) is expressed transiently inflammatory regions in various cardiovascular diseases. We investigated the possibility that specific antibody against TN-C is useful for detecting cardiovascular diseases as a molecular diagnostic reagent. Heavy chain variable region (VH) and light chain variable region (VL) genes of anti-TN-C antibody were obtained from total RNA in hybridoma, and were performed overlap-extended PCR. Expression vector inserted VH-linker-VL gene (scFv) was used in E. coli, in vitro cell free (RTS wheat germ) and mammalian cell line (HEK293) expression systems. As a result, the antibody expression was highly effective in HEK293 cell line system. As soon as it’s ready, we are planning to experiments of antibody accumulation in foci of myocarditis model mice. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2012年度~2014年度科学研究費補助金(若手研究(B))研究成果報告書 | |||||
書誌情報 |
発行日 2015-05-29 |
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フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
Establishment of diagnosis based on molecular targeting imaging of cardiovascular diseases by scFv antibody against Tenascin-C | ||||||
出版者 | ||||||
出版者 | 三重大学 | |||||
科研費番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 24791291 | |||||
資源タイプ(三重大) | ||||||
Kaken / 科研費報告書 |