In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy
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In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major
challenge in metastasis research.We developed a technique for intravital imaging of colorectal liver metastasis development in live
mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM
procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells
were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed
visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated
liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course
imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug
evaluation, reducing the effects of interindividual variation.
In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy
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