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バイオリアクターによる醤油製造法の研究 : 第1報 醤油モデル系による発酵条件の検討 山田, 哲也 Yamada, Tetsuya 小瀬古, 茂樹 Koseko, Shigeki 坪内, 一夫 Tsubouchi, Kazuo 久松, 眞 Hisamatsu, Makoto 498 Soy-sauce production bioreactor fixed yeast ceramic application/pdf Soy‐sauce production by bioreactor was studied. Results are summarized as follows: 1. Selection of yeast fixation material; Among 8 species tested for ability to fixate yeast (Zygosac‐ charomyces rouxii SJ 002),a honeycomb type ceramic(Nippon-gaishi, cordierite) and a bead‐type ceramic (Nippon-sharyo, R - 1) were selected. Their fixation abilty was 4.83 mg/g (wet yeast/ceramic)and 1.71 mg/g (wet yeast/ceramic),respectively. 2. Condition of fementation by bioreactor; Two different bioreactor types were sclected for the fementation‐one was string beads(Tokyo‐rika MBR‐033, total volume; 900 ml), the other type was cylindrical (φ4 cm x 40cm, total volume; 400 mi). The adopted medium for fementation was a modified soy sauce medium (composition; raw soy‐sauce (kiage‐shoyu) 10%, glucose 5%, NaCl (final) 10%). Fermentation was carried out in both systems: string bead reactor type packed with the beads, and the cylinder type packed with honetcombs. Proper aeration(0.1 vvm) allowed for fermentation over one week at 30°C without decreasing the yeast population. 3. Fermentation results: With both reactors, alcohol formed was 2% and residual glucose concen- tration becane 0.56%. This indicates the alcohol conversion ratio to be 80%, and alcohol productivity was calculated to be l.7g/dl h. 4. Practical trial of fermentation by bioreactor; Sardine hydrolyzate solution, which was prepared with protease treatment, was adopted to check the bioreactor systems. The solution, to which 5% glucose was added, was subjected to the string beads type bioreactor in which the yeast had been fixed. Analysis of fermented solution rendered results regarding alcohol formaticn as well as taste evaluation of the solution. The above results suggest that our bioreactor system is favorable to soy souce production and new seasoning using similar production methods. departmental bulletin paper 三重大学生物資源学部 1989-03-30 VoR application/pdf 三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 2 71 79 0915-0471 AN10073846 https://mie-u.repo.nii.ac.jp/record/5689/files/AN100738460000207.pdf http://hdl.handle.net/10076/3040 https://mie-u.repo.nii.ac.jp/records/5689 jpn