2026-03-14T13:55:31Z https://mie-u.repo.nii.ac.jp/oai

oai:mie-u.repo.nii.ac.jp:00008097 2023-10-12T03:00:43Z 631:632:633

In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy Tanaka, Koji Morimoto, Yuhki Toiyama, Yuji Matsushita, Kohei Kawamura, Mikio Koike, Yuhki Okugawa, Yoshinaga Inoue, Yasuhiro Uchida, Keiichi Araki, Toshimitsu Mizoguchi, Akira Kusunoki, Masato application/pdf In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research.We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation. journal article Hindawi Publishing Corporation 2011-11-03 VoR application/pdf Journal of oncology 2012 265487 1687-8469 https://mie-u.repo.nii.ac.jp/record/8097/files/39A15352.pdf http://hdl.handle.net/10076/11796 https://mie-u.repo.nii.ac.jp/records/8097 eng