@article{oai:mie-u.repo.nii.ac.jp:00010172,
 author = {片江, 宏巳 and Katae, Hiromi and 河野, 薫 and Kouno, Kaoru and 森川, 進 and Morikawa, Susumu and 田代, 文男 and Tashiro, Fumio and 高橋, 誓 and Takahashi, Sei and 松丸, 豊 and Matsumaru, Yutaka and 牛山, 宗人 and Ushiyama, Munehiro and 宮崎, 照雄 and Miyazaki, Teruo and 窪田, 三郎 and Kubota, Saburoh S.},
 issue = {2},
 journal = {魚病研究},
 month = {Sep},
 note = {application/pdf, アユ, アマゴおよびウナギを用いてPA の魚体内分布, 残留性および安全性について検討を行ない次の結果を得た。
1. PA は経口投与により魚体内に比較的速やかに吸収され, 組織移行性は良好であった。
2. PA の組織中濃度は魚種により多少異なり, アユはアマゴに比べ各組織中の最高濃度は高い値を示した。 また, アマゴはアユに比べ各組織中濃度は持続的に推移した。
3. アマゴにPA 40mg/kg/日を7 日間連続経口投与した後の組織中濃度の推移は1 回投与後のパターンと類似していた。各組織中のPA 濃度はピークに達した後, 一次式に従って減少し, 特定の臓器または組織に残留または蓄積する傾向はみられなかった。
4. PA の魚類に対する経口毒性は弱く, アユに対するLD₅₀値は2,000 mg/kg 以上であった。また, アユ( 80 mg/kg/日）,　アマゴ（800 mg/kg/日）およびウナギ( 400 mg/kg/日）に5 - 10 日間連続経口投与を行なったが, PA によると思われる死魚はみられず, 臨床所見および組織所見ともに異常は認められなかった。, The pharmacokinetic and toxicological examinations of piromidic acid (PA, 8-ethyl-5, 8-dihydro- 5-oxo-2-pyrrolidinopyrido[2, 3-d]pyrimidine-6-carboxylic acid) were studied in ayu Plecoglossus al-tivelis, amago salmon Oncorhynchus rhodurus and eels Anguilla japonica and A. anguilla orally administered a single or multiple dose(s) of PA with the following results. 
1. The absorption, distribution and excretion data obtained from the bioassay revealed that orally　administered PA was easily absorbed and quickly became distributed in blood and major organs. Peak levels were reached in 3h (in ayu) or 6-24 h (in amago salmon) after the administration of PA, followed by a gradual decrease. The levels of PA in liver and kidney were higher than in blood and plasma. 
2. In acute toxicity study, the LD₅₀ value for ayu was more than 2,000 mg/kg. The subacute toxicity of PA was studied in ayu (80 mg/kg), amago salmon (800 mg/kg) and eels (400 mg/kg) administered PA once daily for 5-10 days. Except for accidental death of two eels, all fishes receiving PA survived without any clinical and histopathological changes. 
3. Disappearance of PA from the tissues in amago salmon was studied after the administration of 40mg/kg of PA for 7 consecutive days. Peak levels of PA in the tissues tested attained within 6-12 h following administration. A level below the assay limits (0.25-0.56μg/ml or g) was reached 96 h post dosing. No evidence of long term residue was found in any of the tissues tested.},
 pages = {79--91},
 title = {ピロミド酸に関する魚病化学療法的研究－Ⅰ. :　魚類における吸収, 分布, 残留性および安全性},
 volume = {14},
 year = {1979}
}