@article{oai:mie-u.repo.nii.ac.jp:00010226,
 author = {宮崎, 照雄 and Miyazaki, Teruo and 窪田, 三朗 and Kubota, Saburoh S.},
 issue = {3},
 journal = {魚病研究},
 month = {Nov},
 note = {application/pdf, 1. V. anguillicida の破壊菌体注射により感作した魚に生菌攻撃を行ない, 感作魚の感染防御反応について病理組織学的に検討した。 2. その結果, 生残率は60%に達し, 生残魚では顕著な好中球反応が観察された。 3. 瀕死魚では好中球反応が弱いながらも認められた。, In this study, Japanese eels were injected with bacterial sonicate and then challenged with viable cells of Vibrio anguillicida. Bacterial sonicate was injected intramuscularly at a rate of 2.0 mg per 100 g body weight. After 24 hours, the treated fishes were challenged with an intramuscular injection of the viable bacteria at rates of 0.5 and 1.0mg per 100 g body weight. Sixty percent of challenged fishes survived for three to seven days. The fishes produced protrudent lesions at the injected areas within three days and ulcerative lesions on the fifth day. In the protrudent lesions, many neutrophils infiltrated and phagocytized bacteria extensively in the affected tissues which exhibited necrosis, edema and hemorrhage. In the viscera, reactions of neutrophils and cells belonging to the reticulo-endothelial system were extensive, which prevented bacterial dissemination. Forty percent of challenged fishes became moribund within two days. The moribund fishes had protrudent lesions at the injected areas and exhibited the congested liver, splenomegaly and enteritis. They underwent systemic infection and the infected tissues exhibited extensive necrosis, circulatory disturbances and slight reactions of neutrophils. Based on the results, it was clarified that the fishes which were previously treated with the bacterial sonicate, manifested extensive reactions of neutrophils as defensive responses against challenge with the viable bacteria.},
 pages = {101--109},
 title = {ニホンウナギのビブリオ病の病理組織学的研究-Ⅱ. : 破壊菌体で前処理した魚の生菌攻撃に対する反応},
 volume = {16},
 year = {1981}
}