{"created":"2023-06-19T11:41:27.445583+00:00","id":10233,"links":{},"metadata":{"_buckets":{"deposit":"a6e1164d-3be6-45f1-8f14-69d1def03c2f"},"_deposit":{"created_by":15,"id":"10233","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10233"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00010233","sets":["420:684:685"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"801","bibliographicPageStart":"793","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Plant physiology and biochemistry"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ni homeostasis is essential for plant cell activity, but the mechanisms of Ni-transport and delivery are unknown. To elucidate the role of ZIP and NRAMP metal-transporters for Ni²⁺-transport and homeostasis, we cloned their homologous genes from the Ni hyperaccumulator Thlaspi japonicum, and investigated their Ni-transporting abilities by expression in yeast. The deduced amino acid sequences of the two Zip transporter genes (TjZnt1, TjZnt2) and one Nramp transporter gene cloned had high homologies with TcZNT1 and TcZNT2 of Thlaspi caerulescens and AtNRAMP4 of Arabidopsis thaliana, respectively, and were predicted as integral membrane proteins with 6 or 12 transmembrane domains. TjZNT1 and TjZNT2 had two long histidine-rich domains in the putative cytoplasmic domain between transmembrane domains III and IV. TjNRAMP4 conserved a consensus transporter motif between transmembrane domains VIII and IX. The yeast transformed with TjZNT1 or TjZNT2 showed a marked increase in Ni²⁺ tolerance with the gene expression. In contrast, the expression of TjNramp4 caused elevation of Ni²⁺ sensitivity and Ni²⁺ concentration. These data suggest that ZIP/NRAMP transporters participate in Ni²⁺ homeostasis of Ni hyperaccumulator plants. TjZNT1 had Zn²⁺-, Cd²⁺- and Mn²⁺-transporting abilities and TjZNT2 also had Zn²⁺- and Mn²⁺-transporting abilities, but TjNRAMP4 could transport Ni²⁺ but not Zn²⁺, Cd²⁺ or Mn²⁺.","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"重金属汚染土壌の浄化と重金属資源の回収技術開発に関する研究","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Gauthier-Villars"}]},"item_10001_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://apps.isiknowledge.com/WoS/CIW.cgi?SID=R29a3ClGpH3a6jdOKGb&Func=Abstract&doc=8/1"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRD-4H0RYX3-2-K&_cdi=6232&_user=1227225&_orig=browse&_coverDate=08%2F31%2F2005&_sk=999569991&view=c&wchp=dGLbVtb-zSkWW&md5=356460f7b8452ec889444152cad98b41&ie=/sdarticle.pdf"}]}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10699073","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0981-9428","subitem_source_identifier_type":"PISSN"}]},"item_10001_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"430","subitem_subject_scheme":"NDC"}]},"item_10001_text_43":{"attribute_name":"著作の一部","attribute_value_mlt":[{"subitem_text_value":"http://hdl.handle.net/10076/7143"}]},"item_10001_text_68":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"出版者版電子ジャーナルあり"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mizuno, Takahumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Usui, Koji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Horie, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nosaka, Shiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mizuno, Naoharu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Obata, Hitoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"50A5789.pdf","filesize":[{"value":"35.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50A5789.pdf","url":"https://mie-u.repo.nii.ac.jp/record/10233/files/50A5789.pdf"},"version_id":"5a74c306-07eb-4e3e-85e6-d17513507cc0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"histidine-rich domain","subitem_subject_scheme":"Other"},{"subitem_subject":"hyperaccumulator","subitem_subject_scheme":"Other"},{"subitem_subject":"Ni-transport","subitem_subject_scheme":"Other"},{"subitem_subject":"Thlaspi japonicum","subitem_subject_scheme":"Other"},{"subitem_subject":"TjNRAMP4","subitem_subject_scheme":"Other"},{"subitem_subject":"TjZNT1","subitem_subject_scheme":"Other"},{"subitem_subject":"TjZNT2","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant thlaspi japonicum and their Ni²⁺ transport abilities","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant thlaspi japonicum and their Ni²⁺ transport abilities","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["685"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-12-20"},"publish_date":"2007-12-20","publish_status":"0","recid":"10233","relation_version_is_last":true,"title":["Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant thlaspi japonicum and their Ni²⁺ transport abilities"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-17T00:31:10.513180+00:00"}