{"created":"2023-06-19T11:41:27.617178+00:00","id":10237,"links":{},"metadata":{"_buckets":{"deposit":"b3236364-be84-4bed-b09c-3fad27606012"},"_deposit":{"created_by":15,"id":"10237","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10237"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00010237","sets":["420:684:685"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-09-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"337","bibliographicPageStart":"329","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Journal of the Japanese Society for Horticultural Science"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Wepreviously found a large increase in RNAs in roots of chill-tolerant figleaf gourds(<special>Cucurbita ficifolia</special> Bouche) but not in those of less tolerant cucumbers (<special>Cucumis sativus</special> L.) when the roots were exposed to 14℃ (Kanda et al., 1994). In this study, changes in ribonuclease and RNA polymerase activities in the roots were followed for 6 days sfter their exposure to 14℃ and 23℃. The root enzyme activities were assayed at a) the optimal temperatures for the enzymes and b) root-growing temperatures. Neither ribonuclease nor RNA polymerase activity accounted for the characteristic increase of RNA concentrations in chilled roots of figleaf gourds, irrespective of assay temperatures. However, the ratio of RNA polymerase activity to ribonuclease activity closely correlated with RNA concentrations in both species. This correlation was observed only when the enzyme activity was assayed at root-growing temperatures. In this case, ribonuclease activity 2 days after exposure to 14℃ was much lower in figleaf gourd roots than that in cucumber roots. However, the activity changed little thereafter in either species, but the RNA polymerase activity in figleaf gourd roots did increase gradually during exposure to 14℃; it was still lower than the activity at 23℃ after 6 days. This increase was not observed in the cucumber roots. These results strongly suggest that, 1) root RNA concentrations in figleaf gourds and cucumbers are largely regulated by RNA polymerase activity relative to ribonuclease activity, and 2) the marked increase of RNA concentrations in figleaf gourd roots grown at 14℃ was caused mainly by low temperature-induced increases of RNA polymerase of RNA polymerase activity together with much reduced activity of ribonuclease.","subitem_description_type":"Abstract"},{"subitem_description":"14℃の低根温遭遇によってクロダネカボチャの根のRNA濃度が著しく高まる機作を明らかにするため，14℃と23℃の根温で6日間生育している間の，クロダネカボチャとキュウリの根のリボヌクレアーゼ活性とRNAポリメラーゼ活性の経時変化を調べた．活性の測定は，それぞれの酵素の適温と採取時の根の生育根温で行った．その結果，いずれの酵素活性も単独では，根温14℃で生育したクロダネカボチャの根でみられたRNA濃度の著しい増大を説明することはできなかった．しかし，リボヌクレアーゼ活性に対するRNAポリメラーゼの活性の比率は，生育根温で活性を測定した場合にのみ，根のRNA濃度と高い相関関係を示した．生育根温で活性を測定した場合，リボヌクレアーゼ活性は，両植物とも23℃で生育した根より14℃で生育した根のほうが低かったが，低下の程度はクロダネカボチャのほうが顕著であった．一方，RNAポリメラーゼ活性は，キュウリでは14℃で生育した根と23℃で生育した根ではほぼ同じであったのに対して，クロダネカボチャでは14℃で生育した根のほうが小さかった．しかし，クロダネカボチャの根では14℃で生育している間に次第に活性が増大した．以上の結果から，両植物の根のRNA濃度は，主として生育根温下での2つの酵素の活性比率に支配されていると考えられる．また，14℃で生育したクロダネカボチャの根におけるRNA濃度の著しい増大は，低温によるリボヌクレアーゼ活性の顕著な低下と，RNAポリメラーゼ活性の低温適応的増大によっていると考えられる．","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"園藝學會"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12177046","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1882-3351","subitem_source_identifier_type":"PISSN"}]},"item_10001_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"460","subitem_subject_scheme":"NDC"}]},"item_10001_text_25":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"低温に遭遇したクロダネカボチャの根のRNA濃度の増大におけるリボヌクレアーゼ活性とRNAポリメラーゼ活性の関与"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KANDA, Hiroomi","creatorNameLang":"en"},{"creatorName":"神田, 啓臣","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"TACHIBANA, Shoji","creatorNameLang":"en"},{"creatorName":"橘, 昌司","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"50A6746.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50A6746.pdf","url":"https://mie-u.repo.nii.ac.jp/record/10237/files/50A6746.pdf"},"version_id":"b7051cc8-0eeb-4884-9bad-45945762932c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Involvement of Ribonuclease and RNA Polymerase Activity in Chill-induced Increases of RNA Concentration in Figleaf Gourd Roots","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of Ribonuclease and RNA Polymerase Activity in Chill-induced Increases of RNA Concentration in Figleaf Gourd Roots","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["685"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-15"},"publish_date":"2008-02-15","publish_status":"0","recid":"10237","relation_version_is_last":true,"title":["Involvement of Ribonuclease and RNA Polymerase Activity in Chill-induced Increases of RNA Concentration in Figleaf Gourd Roots"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-17T00:31:21.903593+00:00"}