{"created":"2023-06-19T11:41:28.636663+00:00","id":10261,"links":{},"metadata":{"_buckets":{"deposit":"e877c6f9-635a-4907-b9d8-37f591020890"},"_deposit":{"created_by":15,"id":"10261","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10261"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00010261","sets":["420:684:685"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Pt24","bibliographicPageEnd":"4410","bibliographicPageStart":"4399","bibliographicVolumeNumber":"210","bibliographic_titles":[{"bibliographic_title":"The journal of experimental biology"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Molluscan smooth muscle can maintain tension over extended periods with little energy expenditure, a process termed catch. Catch is thought to be regulated by phosphorylation of a thick filament protein, twitchin, and involves two phosphorylation sites, D1 and D2, close to the N and C termini, respectively. This study was initiated to investigate the role of the D2 site and its phosphorylation in the catch mechanism. A peptide was constructed containing the D2 site and flanking immunoglobulin (Ig) motifs. It was shown that the dephosphorylated peptide, but not the phosphorylated form, bound to both actin and myosin. The binding site on actin was within the sequence L10 to P29. This region also binds to loop 2 of the myosin head. The dephosphorylated peptide linked myosin and F-actin and formed a trimeric complex. Electron microscopy revealed that twitchin is distributed on the surface of the thick filament with an axial periodicity of 36.25 nm and it is suggested that the D2 site aligns with the myosin heads. It is proposed that the complex formed with the dephosphorylated D2 site of twitchin, F-actin and myosin represents a component of the mechanical linkage in catch.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cambridge University Press"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"18055628","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1242/jeb.008722","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2219545"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://jeb.biologists.org/cgi/content/full/210/24/4399"}]}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00697490","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-0949","subitem_source_identifier_type":"PISSN"}]},"item_10001_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_10001_text_68":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"出版者版電子ジャーナルあり"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Funabara, Daisuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hamamoto, Chieko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Koji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Akinori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ueda, Miki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Osawa, Rika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanoh, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"J., Hartshorne  David","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Suzuki, Suechika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Watabe, Shugo","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"50A7882.pdf","filesize":[{"value":"4.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50A7882.pdf","url":"https://mie-u.repo.nii.ac.jp/record/10261/files/50A7882.pdf"},"version_id":"07d6ee81-ac8d-433b-9bc7-8bd567ffd773"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"catch contraction","subitem_subject_scheme":"Other"},{"subitem_subject":"twitchin","subitem_subject_scheme":"Other"},{"subitem_subject":"ABRM","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin","subitem_subject_scheme":"Other"},{"subitem_subject":"actin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["685"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-11-12"},"publish_date":"2008-11-12","publish_status":"0","recid":"10261","relation_version_is_last":true,"title":["Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-17T02:13:32.935773+00:00"}