@article{oai:mie-u.repo.nii.ac.jp:00010308,
 author = {KIMURA, Taeko and 木村, 妙子 and TABE, Masaaki and 田部, 雅昭},
 issue = {1},
 journal = {Venus : the Japanese journal of malacology},
 month = {Apr},
 note = {application/pdf, Limnoperna fortunei fortunei (Dunker, 1856) and L. fortunei kikuchii Habe, 1981 were introduced in Japan in the 1990's and 1970's, respectively. The latter has been recognized as a subspecies on the basis of features of shell morphology. However, validity of its subspecific status has been questioned, because these two mytilids are clearly distinguished also by several other characteristics. In the present study, genetic differences were examined between these two mytilids, which were collected from two sites in the Nagara River flowing into Ise Bay along the Pacific coast of central Japan. The sample of L. fortunei fortunei was from an upstream site while that of L. fortunei kikuchii was from a downstream site. Examination of these samples for 10 enzymes using gel electrophoresis showed genetic variation to be found at 14 loci, 13 of which indicated complete allelic substitution between the two samples. No hybrid was observed in samples containing the two mytilids. Nei's genetic distance (D) was very high (2.78) between the two. The observed average heterozygosity (Ho) was 0.0048 in L. fortunei fortunei and 0.0783 in L. fortunei kikuchii, the values being significantly different between the samples. These support that their genetic difference would be in the specific level.
付着性二枚貝のカワヒバリガイLimnoperna fortunei fortunei (Dunker, 1856)とその亜種コウロエンカワヒバリガイL. fortunei kikuchii Habe, 1981はいずれもイガイ科カワヒバリガイ属の移入種である。前者は1990年代初めに日本の淡水域に移入し, 後者は1970年代に日本の内湾汽水域に移入した。コウロエンカワヒバリガイは, 殻の形態の違いをもとにカワヒバリガイの亜種とされた。しかし, これらは殻の色彩, 閉殻筋痕, 塩分濃度耐性が明らかに異なり, 別種の可能性が高いと指摘されてきた。今回, 同一河川内のこれら2種類についてアイソザイム分析を行い, 遺伝的分化程度を推定した。試料は岐阜県長良川流域の2地点において採集した。10酵素を用いて分析した結果, 14遺伝子座においてその遺伝様式を推定することができた。遺伝様式の推定できた14遺伝子座のうちAATを除く全ての遺伝子座において2種類間に対立遺伝子の置換がみられた。遺伝的距離はD=2.78と大きく, いずれの遺伝子座においても交雑個体と考えられる泳動像は観察されなかった。これらのことから2種類は別種と考えられる。集団内の遺伝的変異の程度を示す遺伝子多様度は, カワヒバリガイではHo=0.0048なのに対し, コウロエンカワヒバリガイではHo=0.0783とカワヒバリガイの方が有意に小さかった。この理由について考察した。},
 pages = {27--34},
 title = {Large Genetic Differentiation of the Mussels Limnoperna fortunei fortunei (Dunker) and Limnoperna fortunei kikuchii Habe (Bivalvia : Mytilidae)},
 volume = {56},
 year = {1997}
}