{"created":"2023-06-19T11:41:48.397189+00:00","id":10730,"links":{},"metadata":{"_buckets":{"deposit":"710ed1f9-a07e-4260-8716-406cd22afbb0"},"_deposit":{"created_by":15,"id":"10730","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10730"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00010730","sets":["420:724:1692754933579"]},"author_link":["26823","26824"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-05-14","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"海洋細菌Vibrio sp.MA-138のゲノムDNA由来の組換えβ-1.4-マンナン結合タンパク質(CBM)を蛍光標識し、これをプローブとして、アマノリと同じ科に属するウシケノリから単離したプロトプラストを用いて、細胞壁が形成される様子を観察した。その結果、蛍光CBMは12時間培養後のプロトプラストで結合が観察され始め、60時間培養でプロトプラスト全体がβ-1,4-マンナンで覆われることが判明した。","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成19~20年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19580235","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Structural analysis of the cell wall of a red alga, Bangia atropurpurea by using a fluorescent CBM"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"荒木, 利芳","creatorNameLang":"ja"},{"creatorName":"Araki, Toshiyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"苅田, 修一","creatorNameLang":"ja"},{"creatorName":"Karita, Shuichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"50K16605.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50K16605.pdf","url":"https://mie-u.repo.nii.ac.jp/record/10730/files/50K16605.pdf"},"version_id":"6960cb2c-14a0-4ffd-83dc-61b401539c00"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ウシケノリ","subitem_subject_scheme":"Other"},{"subitem_subject":"プロトプラスト","subitem_subject_scheme":"Other"},{"subitem_subject":"β-1.4-マンナナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"CBM","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞壁","subitem_subject_scheme":"Other"},{"subitem_subject":"GFP","subitem_subject_scheme":"Other"},{"subitem_subject":"β-1.3-キシラナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"アガラーゼ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"蛍光ＣＢＭを用いた紅藻アマノリ細胞壁の構造解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"蛍光ＣＢＭを用いた紅藻アマノリ細胞壁の構造解析","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692754933579"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-03-10"},"publish_date":"2014-03-10","publish_status":"0","recid":"10730","relation_version_is_last":true,"title":["蛍光ＣＢＭを用いた紅藻アマノリ細胞壁の構造解析"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-20T04:04:29.418258+00:00"}