{"created":"2023-06-19T11:41:49.021746+00:00","id":10745,"links":{},"metadata":{"_buckets":{"deposit":"c7473208-f3c1-4a8e-95bf-54495f68360a"},"_deposit":{"created_by":15,"id":"10745","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10745"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00010745","sets":["420:724:1692755065618"]},"author_link":["26891"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04-18","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"リグニン由来の機能性バイオ素材「リグノフェノール」を吸着素材として用いるセルラーゼ分離回収,再利用システムを提案し,吸着・脱離性能について検討した。セルラーゼの吸着は,混合撹拌のみで10分以内に飽和吸着量に達し,吸着量は100mg/g(粗酵素ベース)以上に達した。針葉樹を原料とした方が吸着量は大きく,グルコースによる吸着阻害は認められなかった。pH,塩濃度制御による脱離は困難で,有機溶媒添加による沈殿回収では酵素の溶解性に問題が生じた。吸着回収後,固定化セルラーゼとして利用したとき,ネイティブ酵素の約20～60%の活性を維持し,至適pHの拡大が認められた。","subitem_description_type":"Abstract"},{"subitem_description":"Enzymatic hydrolysis of cellulosic biomass by cellulase has been receiving attention. In this study, recovery and recycling of cellulase were proposed using a functional lignin-based phenolic polymer \"\"lignophenol\"\" as an adsorption carrier. Cellulase was easily immobilized on lignophenol simply by mixing to give water-insoluble cellulase-lignophenol complex. Adsorption capacity of lignophenol from softwood was higher than that from hardwood. The presence of glucose did not change the adsorption capacity. Control of pH and salt concentration did not work effectively for desorption of the cellulase. Precipitation separation of cellulase with organic solvents was possible, but the reuse seemed difficult because of limited solubility to water. Cellulase-lignophenol complex was available as an immobilized cellulase with 20-60% of enzymatic activity of native cellulase with wider optimum pH range.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成20~22年度科学研究費補助金(若手研究(B))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"20710059","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Recovery and Recycling of cellulase using a functional biopolymer"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野中, 寛","creatorNameLang":"ja"},{"creatorName":"NONAKA, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-20"}],"displaytype":"detail","filename":"50K16727.pdf","filesize":[{"value":"323.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50K16727.pdf","url":"https://mie-u.repo.nii.ac.jp/record/10745/files/50K16727.pdf"},"version_id":"64604d74-fc4f-4175-989f-aeb9cc7ec9e3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"セルラーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"セルロース","subitem_subject_scheme":"Other"},{"subitem_subject":"酵素糖化","subitem_subject_scheme":"Other"},{"subitem_subject":"吸着回収","subitem_subject_scheme":"Other"},{"subitem_subject":"バイオエタノール","subitem_subject_scheme":"Other"},{"subitem_subject":"リグノフェノール","subitem_subject_scheme":"Other"},{"subitem_subject":"リグニン","subitem_subject_scheme":"Other"},{"subitem_subject":"固定化酵素","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"機能性バイオ素材を用いたセルラーゼの分離回収およびリサイクル利用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"機能性バイオ素材を用いたセルラーゼの分離回収およびリサイクル利用","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692755065618"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-03-10"},"publish_date":"2014-03-10","publish_status":"0","recid":"10745","relation_version_is_last":true,"title":["機能性バイオ素材を用いたセルラーゼの分離回収およびリサイクル利用"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-20T04:40:35.793184+00:00"}