@techreport{oai:mie-u.repo.nii.ac.jp:00010767,
 author = {粟冠, 和郎 and SAKKA, Kazuo and 粟冠, 真紀子 and SAKKA, Makiko},
 month = {May},
 note = {application/pdf, 高温性堆肥化過程において堆肥上層部で90℃以上に達した。培養法による生菌数は、顕微鏡法による生菌数の1/100～1/1000であり、難培養性細菌の重要性を示した。堆肥化開始後14日目の優占菌はBacillus属細菌であり36％を占めたが、81日目にはMelghirimyces thermohalophilusが63％を占め、菌叢は大きく変化した。堆肥中のDNAから直接好熱性酵素遺伝子が単離されたこと、好熱性細胞壁分解酵素生産菌が分離されたことから、高温性堆肥が遺伝子資源として有用であることが示された。, Temperature reached over 90 degrees centigrade in the upper position of the compost of the high-temperature composting system. Viable cell numbers by surface plate method was about 1/100 to 1/1000 of those by microscopic observation method, suggesting that unculturable bacteria were important in the high-temperature composting process. Bacillus species dominated in the early stage of composting, accounting for 36% of the total cell numbers, but Melghirimyces thermohalophilus accounted for 63%, indicating that bacterial flora drastically changed in the process of the composting. Genes encoding thermophilic enzymes were directly cloned from DNA isolated from the compost and thermophilic bacteria producing bacterial cell wall-degrading enzymes were isolated from the compost, indicating that the high-temperature compost was quite useful as the genetic resource for isolating thermophilic enzymes and bacteria., 2012年度～2013年度科学研究費補助金(挑戦的萌芽研究)研究成果報告書, 24658075},
 title = {高温性堆肥の微生物生態と遺伝子資源としての有用性},
 year = {2014}
}