{"created":"2023-06-19T11:42:12.489846+00:00","id":11311,"links":{},"metadata":{"_buckets":{"deposit":"4b883628-4fdf-43bc-87b3-f6c05b231779"},"_deposit":{"created_by":16,"id":"11311","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"11311"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00011311","sets":["334:608:618:833"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Because existing therapeutic cancer vaccines provide only a limited clinical benefit, a different vaccination strategy is necessary to improve vaccine efficacy. We developed a nanoparticulate cancer vaccine by encapsulating a synthetic long peptide antigen within an immunologically inert nanoparticulate hydrogel (nanogel) of cholesteryl pullulan (CHP). After subcutaneous injection to mice, the nanogel-based vaccine was efficiently transported to the draining lymph node, and was preferentially engulfed by medullary macrophages but was not sensed by other macrophages and dendritic cells (so-called “immunologically stealth mode”). Although the function of medullary macrophages in T cell immunity has been unexplored so far, these macrophages effectively cross-primed the vaccine-specific CD8＋ T cells in the presence of a Toll-like receptor (TLR) agonist as an adjuvant. The nanogelbased vaccine significantly inhibited in vivo tumor growth in the prophylactic and therapeutic settings, compared to another vaccine formulation using a conventional delivery system, incomplete Freund's adjuvant. We also revealed that lymph node macrophages were highly responsive to TLR stimulation, which may underlie the potency of the macrophage-oriented, nanogel-based vaccine. These results indicate that targeting medullary macrophages using the immunologically stealth nanoparticulate delivery system is an effective vaccine strategy.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文 / Department of Immuno-Gene Therapy, Mie University Graduate School of Medicine","subitem_description_type":"Other"},{"subitem_description":"10p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第1757号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/nn502975r","subitem_relation_type_select":"DOI"}}]},"item_7_relation_37":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://pubs.acs.org/doi/abs/10.1021/nn502975r"}]}]},"item_7_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2014 American Chemical Society"}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"ACS Nano,  8 (9), 2014, pp 9209-9218 ISSN : 1936-086X に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Muraoka, Daisuke","creatorNameLang":"en"},{"creatorName":"ムラオカ, ダイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"村岡, 大輔","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-07-04"}],"displaytype":"detail","filename":"2015DM020.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2015DM020","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/11311/files/2015DM020.pdf"},"version_id":"4f7582fa-4465-4aed-b372-8ac8db5b6aad"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cancer vaccine","subitem_subject_scheme":"Other"},{"subitem_subject":"nanogel","subitem_subject_scheme":"Other"},{"subitem_subject":"vaccine delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"macrophages","subitem_subject_scheme":"Other"},{"subitem_subject":"lymph node","subitem_subject_scheme":"Other"},{"subitem_subject":"T cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["833"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-07-04"},"publish_date":"2017-07-04","publish_status":"0","recid":"11311","relation_version_is_last":true,"title":["Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-11-06T04:42:42.790833+00:00"}