{"created":"2023-06-19T11:42:13.127616+00:00","id":11326,"links":{},"metadata":{"_buckets":{"deposit":"3c1813d7-5741-495a-9f5f-2c11023fcad6"},"_deposit":{"created_by":16,"id":"11326","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"11326"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00011326","sets":["334:608:618:833"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Pemetrexed transport by human organic anion transporters, hOAT1 (SLC22A6) and hOAT3 (SLC22A8), were characterized in comparison with methotrexate. Methods: Accumulation of pemetrexed and methotrexate in hOAT1- and hOAT3-expressing cells were evaluated. Pemetrexed and methotrexate were determined by HPLC. Kinetic parameters were calculated by Eadie-Hofstee plot. Results: When HEK-hOAT3 and -hOAT1 cells were incubated with 100 μM pemetrexed for 30min, pemetrexed was accumulated at 14- and 1.7-fold greater than that in control cells, respectively. Pemetrexed and methotrexate transport by hOAT3 was saturated at high concentrations with apparent Km values 28.2 μM and 76.6 μM, respectively. In addition, intrinsic activity (Vmax/Km) of pemetrexed and methotrexate transport by hOAT3 was 4.82 and 0.42 μl/min/mg protein, respectively, suggesting 11-fold higher transport of pemetrexed than methotrexate by hOAT3. Furthermore, loxoprofen, ibuprofen, pravastatin, and cefazolin, transport substrates of hOAT3, inhibited pemetrexed transport by hOAT3 with IC50 values, 34.2, 27.9, 76.3 and 650 μM, respectively. Conclusions: Pemetrexed is a superior substrate to methotrexate for hOAT3. Loxoprofen, ibuprofen, and cefazolin could cause drug-drug interactions when attaining high blood concentrations.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文 / Department of Clinical Pharmacy and Biopharmaceutics, Mie University Graduate School of Medicine","subitem_description_type":"Other"},{"subitem_description":"6p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙医学第993号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2133/dmpk.DMPK-13-RG-042","subitem_relation_type_select":"DOI"}}]},"item_7_relation_37":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://doi.org/10.2133/dmpk.DMPK-13-RG-042"}]}]},"item_7_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2014 by the Japanese Society for the Study of Xenobiotics (JSSX)"}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Drug Metabolism and Pharmacokinetics, 29 (2), 2014, pp.  148-153, ISSN : 1347-4367 に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KURATA, Tomohiko","creatorNameLang":"en"},{"creatorName":"クラタ, トモヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"倉田, 朋彦","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-07-04"}],"displaytype":"detail","filename":"2015DM040.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2015DM040","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/11326/files/2015DM040.pdf"},"version_id":"319ba172-648f-4557-9269-ac3602f94a32"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"pemetrexed","subitem_subject_scheme":"Other"},{"subitem_subject":"organic anion transporter","subitem_subject_scheme":"Other"},{"subitem_subject":"methotrexate","subitem_subject_scheme":"Other"},{"subitem_subject":"renal tubular secretion","subitem_subject_scheme":"Other"},{"subitem_subject":"drug interaction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Characteristics of Pemetrexed Transport by Renal Basolateral Organic Anion Transporter hOAT3","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characteristics of Pemetrexed Transport by Renal Basolateral Organic Anion Transporter hOAT3","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["833"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-07-04"},"publish_date":"2017-07-04","publish_status":"0","recid":"11326","relation_version_is_last":true,"title":["Characteristics of Pemetrexed Transport by Renal Basolateral Organic Anion Transporter hOAT3"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-11-06T04:43:20.293035+00:00"}