{"created":"2023-06-19T11:42:18.717652+00:00","id":11470,"links":{},"metadata":{"_buckets":{"deposit":"80bb6be5-8628-4e32-ae32-93e50ac6b3d3"},"_deposit":{"created_by":16,"id":"11470","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"11470"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00011470","sets":["334:608:618:843"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-24","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-24"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"LFA-1 (aLb2) and Mac-1 (aMb2) integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells. Here we have shown that the anticoagulant molecule thrombomodulin (TM), found on the surface of endothelial cells, functions as a potentially new ligand for leukocyte integrins. We generated a recombinant extracellular domain of human TM and Fc fusion protein (TM-domains 123-Fc), and showed that pheripheral blood mononuclear cells (PBMCs) bind to TM-domains 123-Fc dependent upon integrin activation. We then demonstrated that aL integrin-blocking mAb, aM integrin-blocking mAb, and b2 integrin-blocking mAb inhibited the binding of PBMCs to TM-domains 123-Fc. Furthermore, we show that the serine/threonine-rich domain (domain 3) of TM is required for the interaction with the LFA-1 (aLb2) and Mac-1 (aMb2) integrins to occur on PBMCs. These results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to TM, thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. In fact, integrin-TM interactions might be involved in the dynamic regulation of leukocyte adhesion with endothelial cells.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文 / Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine; Emergency and Critical Care Center, Mie University Hospital","subitem_description_type":"Other"},{"subitem_description":"8p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第1814号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2016.04.007","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Biochemical and Biophysical Research Communications, 473(4), 2016, pp. 1005-1012, ISSN:0006-291X に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawamoto, Eiji","creatorNameLang":"en"},{"creatorName":"カワモト, エイジ","creatorNameLang":"ja-Kana"},{"creatorName":"川本, 英嗣","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-07"}],"displaytype":"detail","filename":"2016DM021.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2016DM021","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/11470/files/2016DM021.pdf"},"version_id":"21cced30-3673-4d45-b1ae-3532c17eea55"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Integrin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thrombomodulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Adhesion molecules","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["843"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-11-07"},"publish_date":"2017-11-07","publish_status":"0","recid":"11470","relation_version_is_last":true,"title":["LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-11-02T05:34:23.967131+00:00"}