{"created":"2023-06-19T11:42:30.708294+00:00","id":11755,"links":{},"metadata":{"_buckets":{"deposit":"f6367bf4-062d-4807-a3ef-cc8591f48fb8"},"_deposit":{"created_by":15,"id":"11755","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"11755"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00011755","sets":["631:636:1692684709779"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-05-29","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"静脈内投与された抗癌剤が癌組織内または細胞内に取り込まれて作用する過程を生体内リアルタイムイメージングするためのモデルの確立を目指し、in vitroおよびin vivoでの実験を行った。抗癌剤として分子標的抗体医薬の抗EGFR抗体や抗c-MET抗体を蛍光標識し、シャーレ上に生着している癌細胞との結合が蛍光顕微鏡で可視化できることを確認した（in vitro）。次に、マウス癌転移モデルを用い蛍光標識した抗EGFR抗体や抗c-MET抗体を静脈内投与すると、癌転移巣で蛍光標識抗体が癌細胞と結合するのが二光子励起顕微鏡で観察できた（in vivo）。","subitem_description_type":"Abstract"},{"subitem_description":"We developed a method of in vivo optical pathology using a multiphoton microscopy (MPM) on metastatic tumor xenograft model (colorectal liver metastasis and gastric peritoneal metastasis), and evaluated chemotherapeutics efficacy in the tumor microenvironment. We imaged the binding of Alexa Fluor labeling anti-epidermal growth factor receptor (EGFR) antibody (cetuximab) to EGFR on viable HT29 cells (colorectal cancer cell line) in vitro without cell fixation. Similarly, we imaged the binding of Alexa Fluor labeling anti- c-MET (hepatocyte growth factor receptor) antibody to MET on viable NUGC4 cells (gastric cancer cell line). We imaged the binding of fluorescently labeled antibodies on tumor cells via their antigens on the metastatic tumor xenografts using a MPM in vivo real-time. In vivo imaging system of dynamics of such drugs.on the metastatic tumor xenografts will be useful to clarify in vivo pharmacokinetic and pharmacodynamic mechanisms.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2013年度～2015年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"25462052","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 光司","creatorNameLang":"ja"},{"creatorName":"タナカ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Koji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"問山, 裕二","creatorNameLang":"ja"},{"creatorName":"トイヤマ, ユウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Toiyama, Yuji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"井上, 靖浩","creatorNameLang":"ja"},{"creatorName":"イノウエ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Inoue, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"内田, 恵一","creatorNameLang":"ja"},{"creatorName":"ウチダ, ケイイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Uchida, Keiichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"毛利, 靖彦","creatorNameLang":"ja"},{"creatorName":"モウリ, ヤスヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Mohri, Yasuhiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"溝口, 明","creatorNameLang":"ja"},{"creatorName":"ミゾグチ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"Mizoguchi, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"楠, 正人","creatorNameLang":"ja"},{"creatorName":"クスノキ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"Kusunoki, Masato","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-12"}],"displaytype":"detail","filename":"39K18182.pdf","filesize":[{"value":"458.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"39K18182","url":"https://mie-u.repo.nii.ac.jp/record/11755/files/39K18182.pdf"},"version_id":"d572be68-3adf-4d90-9319-3a03ed88634d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"二光子励起顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"癌転移","subitem_subject_scheme":"Other"},{"subitem_subject":"生体内リアルタイムイメージング","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"二光子励起顕微鏡を用いた癌転移巣微小環境生体内可視化による薬物動態評価法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"二光子励起顕微鏡を用いた癌転移巣微小環境生体内可視化による薬物動態評価法の開発","subitem_title_language":"ja"},{"subitem_title":"In vivo imaging system of dynamics of molecularly targeted drugs on the metastatic tumor xenografts using a multiphoton microscopy","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692684709779"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-12"},"publish_date":"2018-01-12","publish_status":"0","recid":"11755","relation_version_is_last":true,"title":["二光子励起顕微鏡を用いた癌転移巣微小環境生体内可視化による薬物動態評価法の開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-13T01:57:44.290888+00:00"}