{"created":"2023-06-19T11:42:31.013588+00:00","id":11762,"links":{},"metadata":{"_buckets":{"deposit":"18db147e-c21d-420b-a92b-91c61ba44114"},"_deposit":{"created_by":15,"id":"11762","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"11762"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00011762","sets":["366:680:1692685140697"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-05-26","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"取得したい抗体を発現するB細胞をその抗体に対する抗原を発現させたミエローマ細胞で選択し，両細胞を電気パルスによって選択融合を行い、膜受容体の立体構造を認識することのできる高親和性抗体を産生するハイブリドーマを作製することができた．また，誘電泳動による細胞の操作技術を用いて，迅速で簡便に異なる種類の細胞をマイクロウェルアレイ電極内に導入し，1対1の異種細胞ペアを作製できた．約1分で10万個以上の異種細胞ペアを作製できた．さらに，この細胞ペアに電気パルスを印加すると細胞融合によりハイブリッド細胞を得ることができた．","subitem_description_type":"Abstract"},{"subitem_description":"B cells harboring aimed specific antigen-receptors were captured by antigen-expressing myeloma cells and selectively fused by electrical pulses to generate hybridoma cells secreting stereospecific monoclonal antibodies. By using this method, hybridoma cells can be fabricated to produce the stereospecific structure of the specific receptors on the cell membrane. Cell manipulation technique based on the dielectrophoresis was utilized to introduce the cells to the microwell array electrode and form single cell pairs with different types of cells rapidly and simply. Thus, the use of the present dielectrophoretic manipulation allows large numbers of cell pairs (over 100,000 pairs) to be produced within only 1 min.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2012年度～2015年度科学研究費補助金(基盤研究(B))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"24360339","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"冨田, 昌弘","creatorNameLang":"ja"},{"creatorName":"トミタ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"TOMITA, Masahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"水谷, 文雄","creatorNameLang":"ja"},{"creatorName":"ミズタニ, フミオ","creatorNameLang":"ja-Kana"},{"creatorName":"MIZUTANI, Fumio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"安川, 智之","creatorNameLang":"ja"},{"creatorName":"ヤスカワ, トモユキ","creatorNameLang":"ja-Kana"},{"creatorName":"YASUKAWA, Tomoyuki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-12"}],"displaytype":"detail","filename":"40K18192.pdf","filesize":[{"value":"646.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"40K18192","url":"https://mie-u.repo.nii.ac.jp/record/11762/files/40K18192.pdf"},"version_id":"133989e1-9875-4228-8fe7-266e384d6491"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"B細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"抗原発現細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ハイブリドーマ","subitem_subject_scheme":"Other"},{"subitem_subject":"立体構造特異的抗体","subitem_subject_scheme":"Other"},{"subitem_subject":"誘電泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞配列","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫捕捉","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞分離","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"抗体医薬をめざした最先端ハイブリドーマテクノロジーの開発と応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"抗体医薬をめざした最先端ハイブリドーマテクノロジーの開発と応用","subitem_title_language":"ja"},{"subitem_title":"Advanced hybridoma technology for generating next generation of therapeutic medicines","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692685140697"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-12"},"publish_date":"2018-01-12","publish_status":"0","recid":"11762","relation_version_is_last":true,"title":["抗体医薬をめざした最先端ハイブリドーマテクノロジーの開発と応用"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-17T04:51:07.281940+00:00"}