{"created":"2023-06-19T11:43:17.925191+00:00","id":12787,"links":{},"metadata":{"_buckets":{"deposit":"578154c9-683f-4c3b-bc17-ff0b9932afab"},"_deposit":{"created_by":16,"id":"12787","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"12787"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00012787","sets":["334:608:618:915"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Pirfenidone (PFD), which is an anti-fibrotic agent used for treatment of idiopathic pulmonary fibrosis (IPF), induces G0/G1 cell cycle arrest in fibroblasts. We hypothesized that PFD induced G0/G1 cell cycle arrest might be achieved in other types of cells, including cancer cells. Here we investigated the effects of PFD on the proliferation of pancreatic cancer cells (PCCs) in vitro. Method: Human skin fibroblasts ASF-4-1 cells and human prostate stromal cells PrSC were used as fibroblasts. PANC-1, MIA PaCa-2 and BxPC-3 cells were used as human PCCs. Cell cycle and apoptosis were analyzed using flow cytometer. Results: First, we confirmed that PFD suppressed cell proliferation of ASF-4-1 cells and PrSC, and induced G0/G1 cell cycle arrest. Under these experimental conditions, PFD also suppressed cell proliferation and induced G0/G1 cell cycle arrest in all PCCs. In PFD-treated PCCs, expression of p21 was increased but that of CDK2 was not clearly decreased. Of note, PFD did not induce significant apoptosis among PCCs. Conclusions: These results demonstrated that the anti fibrotic agent PFD might have anti-proliferative effects on PCCs by inducing G0/G1 cell cycle arrest. This suggests that PFD may target not only fibroblasts but also PCCs in the tumor microenvironment of pancreatic cancer.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Mie University, Department of Oncologic Pathology, Tsu-city, (Mie,) Japan","subitem_description_type":"Other"},{"subitem_description":"22p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第1944号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Usugi, Eri","creatorNameLang":"en"},{"creatorName":"ウスギ, エリ","creatorNameLang":"ja-Kana"},{"creatorName":"臼杵, 恵梨","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-16"}],"displaytype":"detail","filename":"2018DM0330.pdf","filesize":[{"value":"809.6 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2018DM0330.pdf","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/12787/files/2018DM0330.pdf"},"version_id":"f3ceb605-80c1-4b49-8a6b-8639f95a5a81"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pirfenidone","subitem_subject_scheme":"Other"},{"subitem_subject":"Anti-proliferative effects","subitem_subject_scheme":"Other"},{"subitem_subject":"Pancreatic cancer cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblasts","subitem_subject_scheme":"Other"},{"subitem_subject":"G0/G1 cell cycle arrest","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Anti-fibrotic agent pirfenidone suppresses 1 proliferation of human pancreatic cancer cells by inducing G0/G1 cell cycle arrest","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-fibrotic agent pirfenidone suppresses 1 proliferation of human pancreatic cancer cells by inducing G0/G1 cell cycle arrest","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["915"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-10-16"},"publish_date":"2019-10-16","publish_status":"0","recid":"12787","relation_version_is_last":true,"title":["Anti-fibrotic agent pirfenidone suppresses 1 proliferation of human pancreatic cancer cells by inducing G0/G1 cell cycle arrest"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-31T06:02:19.834779+00:00"}