{"created":"2023-06-19T11:43:18.140970+00:00","id":12792,"links":{},"metadata":{"_buckets":{"deposit":"d1b3dc7f-e719-4369-9ac8-3c0eeadef7ab"},"_deposit":{"created_by":16,"id":"12792","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"12792"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00012792","sets":["334:608:618:915"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The E2A-HLF oncogenic fusion gene, generated by t(17;19)(q22;p13) translocation in childhood B-cell acute lymphoblastic leukemia with a very poor prognosis, encodes a chimeric transcription factor in which the transactivation domains of immunoglobulin enhancer-binding factor (E2A) are fused to the DNA-binding and dimerization domain of hepatic leukemia factor (HLF). E2A-HLF was shown to have an anti-apoptotic effect. However, the molecular mechanism underlying E2A-HLF-mediated leukemogenesis remains unclear. We identified the transcriptional coactivator Eya2, the forced expression of which is known to immortalize mouse hematopoietic stem/progenitor cells (HSPCs), as a direct target molecule downstream of E2A-HLF. E2A-HLF-immortalized mouse HSPCs expressed Eya2 at a high level in the aberrant self-renewal program. Chromatin immunoprecipitation-quantitative polymerase chain reaction and a reporter assay revealed that E2A-HLF enhanced the Eya2 expression by binding to the promoter region containing the E2A-HLF-binding consensus sequence. Eya2 knockdown in E2A-HLF-immortalized cells resulted in reduced colony-forming efficiency. These results suggest a critical role of Eya2 in E2A-HLF-mediated leukemogenesis.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Microbiology and Molecular Genetics, Mie University Graduate School of Medicine,","subitem_description_type":"Other"},{"subitem_description":"27p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第1953号"}]},"item_7_full_name_2":{"attribute_name":"著者（ヨミ）","attribute_value_mlt":[{"names":[{"name":"マハラジャン, ビスヌ デビ","nameLang":"ja"}]}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MAHARJAN, BISHNU DEVI","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-16"}],"displaytype":"detail","filename":"2018DM0348.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2018DM0348.pdf","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/12792/files/2018DM0348.pdf"},"version_id":"84e6c062-9cd0-4983-872b-ec72f2d2e63b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Eya2","subitem_subject_scheme":"Other"},{"subitem_subject":"E2A-HLF","subitem_subject_scheme":"Other"},{"subitem_subject":"leukemia","subitem_subject_scheme":"Other"},{"subitem_subject":"self-renewal","subitem_subject_scheme":"Other"},{"subitem_subject":"immortalization","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Eya2 is critical for the E2A-HLF-mediated immortalization of mouse hematopoietic stem/progenitor cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Eya2 is critical for the E2A-HLF-mediated immortalization of mouse hematopoietic stem/progenitor cells","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["915"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-10-16"},"publish_date":"2019-10-16","publish_status":"0","recid":"12792","relation_version_is_last":true,"title":["Eya2 is critical for the E2A-HLF-mediated immortalization of mouse hematopoietic stem/progenitor cells"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-31T06:02:31.402201+00:00"}