@phdthesis{oai:mie-u.repo.nii.ac.jp:00013206,
 author = {Tomita, Hisao and 富田, 寿男},
 month = {Jul},
 note = {application/pdf, For a resolution of reducing carbon dioxide emission and increasing food production to respond to the growth of global population, the production of biofuels from non-edible biomass is urgently required. Abundant agricultural wastes, such as orange wastes in orange juice factories and sugar beet pulp (SBP) from sugar refining factories, occur, but they are largely disposed of. These agricultural wastes are non-edible biomass and are able to be utilized as a raw material of second-generation biofuels. This study evaluates the isopropanol-butanol-ethanol (IBE) fermentation ability of Clostridium beijerinckii and cellulosic biomass degrading ability of Clostridium cellulovorans under different concentrations of limonene, which has extremely toxic to fermenting microorganism. As a result, we found that C. cellulovorans was able to grow even in medium containing 0.05% limonene (v/v) and degraded 85% of total sugar from mandarin peel and strained lees without any pretreatments. More interestingly, C. beijerinckii produced 0.046 g butanol per 1 g of dried strained lees in the culture supernatant together with C. cellulovorans. Furthermore, this study indicated that a co-culture fermentation system combining C. cellulovorans with microbial flora of methane production (MFMP), using sugar beet pulp (SBP) as a carbon source, is effective for the direct conversion of cellulosic biomass to methane (CH4). The MFMP was collected from a methane fermenter in commercial operation and was analyzed by a next-generation sequencing system. The microbiome was identified and classified based on several computer programs, revealing that the MFMP included methanogenic organisms such as, Methanosarcina mazei, Methanosaetaceae, Methanosaeta and Methanospirillaceae. Furthermore, Although the MFMP did not degrade SBP, the consortium of C. cellulovorans with MFMP (CCeM) degraded 87.3% of SBP without any pretreatment and produced 34.0 L of CH4 per 1 kg of dry weight of SBP. These results indicated that agricultural wastes can be degraded and converted to CH4 simultaneously by C. cellulovorans and the MFMP., 本文/Graduate School of Bioresources Mie University, 93p},
 school = {三重大学},
 title = {Studies on biofuel production from agricultural wastes by using Clostridium cellulovorans},
 year = {2019},
 yomi = {トミタ, ヒサオ}
}