{"created":"2023-06-19T11:43:38.598197+00:00","id":13262,"links":{},"metadata":{"_buckets":{"deposit":"eebd42d6-e9f7-4eb2-b339-cdb8c776a890"},"_deposit":{"created_by":15,"id":"13262","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"13262"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00013262","sets":["334:627:1692670441288"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-22","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究は、スフェロイドに微小環境が加わった前立腺癌細胞の新組織切片培養で、発現が増強される遺伝子群よりKIF22遺伝子が抽出され，その機能的解析及び診断・治療への応用展開を目的とした。いずれの細胞株においても、細胞成長は抑制され、G2/M期に停止し、またアポトーシスも増加することが認められた。２Dおよびスフェロイド培養において、KIF22遺伝子に対するsiRNA併用による抗癌剤（ドセタキセル）の効果増強を確認することができた。これらの結果により、KIF22遺伝子の前立腺癌への関与および治療への応用の可能性を示すことが出来た。","subitem_description_type":"Abstract"},{"subitem_description":"In our previous study, the prostate cancer-related genes were identified in the new tissue culture system. Among these genes showing enhanced expression in the new tissue culture system, the KIF22 gene was extracted as one of candidate genes related with prostate cancer behavior. In this study, we analyzed proliferation, apoptosis, and cell cycle of the prostate cancer cells (DU-145 and LNCaP) treated with KIF22 siRNA. The inhibition of KIF22 mRNA using siRNA affected cell proliferation in spheroids and monolayers of both cancer cell lines. This suppression also caused G2/M arrest and apoptosis in both cell lines. These results suggest involvement of the KIF22 gene in prostate cancer behavior.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2015年度～2017年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"15K10580","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邉, 昌俊","creatorNameLang":"ja"},{"creatorName":"ワタナベ, マサトシ","creatorNameLang":"ja-Kana"},{"creatorName":"WATANABE, Masatoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"白石, 泰三","creatorNameLang":"ja"},{"creatorName":"シライシ, タイゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIRAISHI, Taizo","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-21"}],"displaytype":"detail","filename":"2019RP0029.pdf","filesize":[{"value":"180.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2019RP0029","url":"https://mie-u.repo.nii.ac.jp/record/13262/files/2019RP0029.pdf"},"version_id":"36b4337b-1f41-4778-9229-8a09570b7a75"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"前立腺癌","subitem_subject_scheme":"Other"},{"subitem_subject":"３次元培養","subitem_subject_scheme":"Other"},{"subitem_subject":"抗癌剤","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新組織切片培養で誘導された前立腺癌関連遺伝子の機能的解析と診断・治療への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新組織切片培養で誘導された前立腺癌関連遺伝子の機能的解析と診断・治療への応用","subitem_title_language":"ja"},{"subitem_title":"Functional analysis of the prostate cancer-related genes obtained from the new tissue culture system","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670441288"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-21"},"publish_date":"2020-01-21","publish_status":"0","recid":"13262","relation_version_is_last":true,"title":["新組織切片培養で誘導された前立腺癌関連遺伝子の機能的解析と診断・治療への応用"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-09T02:01:19.890126+00:00"}