{"created":"2023-06-19T11:43:38.639919+00:00","id":13263,"links":{},"metadata":{"_buckets":{"deposit":"970c9160-aa41-4464-8013-ff2b26de08de"},"_deposit":{"created_by":15,"id":"13263","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"13263"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00013263","sets":["334:627:1692670441288"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-18","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"前立腺の上皮細胞は平滑筋細胞からのパラクライン刺激により形態的・機能的に成熟した分化状態を維持している。しかし、間質リモデリングが生じると上皮細胞の分化状態が二次的に破綻し、前立腺癌の発生に寄与すると考えられる。ヒト前立腺癌患者由来線維芽細胞との共培養によりヒト前立腺幹・前駆細胞モデルBPH-1における癌抑制遺伝子GSTP1 mRNA発現量は有意に低下した。また、去勢により誘導される間質リモデリング周囲の管腔構造が崩壊したエリアにおいてもGSTP1 mRNA発現量は低値を示した。間質リモデリングの誘導が上皮細胞における癌抑制遺伝子発現を減少させ、前立腺癌の発生に寄与している可能性が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Deregulation of epithelial-stromal interactions is considered to play a critical role in the initiation of prostate cancer (PCa). Fibroblasts secret a number of growth factors, cytokines, and miRNAs that affect cellular differentiation of epithelial cells. In this study, we investigated the effects of fibroblasts on the expression of tumor suppressor genes in non-transformed human prostatic epithelial cell line BPH-1 in vitro and in vivo. In in vitro co-culture experiments, mRNA expression of GSTP1 in BPH-1 cells was decreased by co-culturing with fibroblasts. In in vivo co-inoculation experiments, mRNA expression of GSTP1 was quite low in infiltrating solid branching epithelial cords surrounding tenascin-C-expressing fibroblasts. Our data showed that GSTP1 in BPH-1 cells were highly disturbed by interactions with fibroblasts. The use of PCa patients-derived fibroblasts may allow us to investigate the characteristics of aggressive fibroblasts in the initiation of PCa.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2015年度～2017年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"15K10583","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"有馬, 公伸","creatorNameLang":"ja"},{"creatorName":"アリマ, キミノブ","creatorNameLang":"ja-Kana"},{"creatorName":"Arima, Kiminobu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"白石, 泰三","creatorNameLang":"ja"},{"creatorName":"シライシ, タイゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiraishi, Taizo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"石井, 健一朗","creatorNameLang":"ja"},{"creatorName":"イシイ, ケンイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Ishii, Kenichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"佐々木, 豪","creatorNameLang":"ja"},{"creatorName":"ササキ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Sasaki, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"加藤, 学","creatorNameLang":"ja"},{"creatorName":"カトウ, マナブ","creatorNameLang":"ja-Kana"},{"creatorName":"Kato, Manabu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-21"}],"displaytype":"detail","filename":"2019RP0030.pdf","filesize":[{"value":"202.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2019RP0030","url":"https://mie-u.repo.nii.ac.jp/record/13263/files/2019RP0030.pdf"},"version_id":"f94820de-9feb-4c50-bee5-94be829fe465"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"前立腺","subitem_subject_scheme":"Other"},{"subitem_subject":"疾患関連リプログラミング","subitem_subject_scheme":"Other"},{"subitem_subject":"間質リモデリング","subitem_subject_scheme":"Other"},{"subitem_subject":"線維芽細胞の多様性","subitem_subject_scheme":"Other"},{"subitem_subject":"前立腺癌患者由来線維","subitem_subject_scheme":"Other"},{"subitem_subject":"芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"前立腺幹・前駆細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro共培養実験","subitem_subject_scheme":"Other"},{"subitem_subject":"in vivo共移植実験","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"疾患関連リプログラミングによる前立腺癌不均一性の獲得機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"疾患関連リプログラミングによる前立腺癌不均一性の獲得機構","subitem_title_language":"ja"},{"subitem_title":"Mechanisms of prostate cancer heterogeneity induced by disease-associated reprogramming","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670441288"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-21"},"publish_date":"2020-01-21","publish_status":"0","recid":"13263","relation_version_is_last":true,"title":["疾患関連リプログラミングによる前立腺癌不均一性の獲得機構"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-09T02:01:20.253944+00:00"}