{"created":"2023-06-19T11:43:39.645344+00:00","id":13287,"links":{},"metadata":{"_buckets":{"deposit":"7c6c954c-cd4e-4bad-95a8-0a17ac0fd768"},"_deposit":{"created_by":15,"id":"13287","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"13287"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00013287","sets":["631:636:1692684750464"]},"author_link":["43307","43308"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-24","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"今回の研究により、Phosphodiesterase(PDE)の一つであるPDE1の阻害剤が悪性黒色腫細胞の運動能を抑制する機構について解明した。当科で樹立、継代しているヒト口腔悪性黒色腫細胞MAAにsildenafilを作用させると、細胞の先導端においてアクチンの重合が抑制され、lamellipodia(膜状突起)の形成が阻害されていた。MAA細胞はPDE1を発現しPDE5を発現していないことから、sildenafilによるMAAの運動能の抑制には、PDE1の阻害を介した細胞内のcAMP濃度の上昇とlamellipodiaの阻害が関与する可能性が示唆された。\nIn this study, we elucidated the mechanism that PDE1 inhibitor suppresses mobility of malignant melanoma cells. Sildenafil inhibited the polymerization of actin at the leading edge of the human oral malignant melanoma MAA cells, and also inhibited the formation of lamellipodia. Since MAA cells express PDE1 and do not express PDE5, the elevation of intracellular cAMP concentration through inhibition of PDE1 may be involved in suppression of the mobility of MAA by sildenafil.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2015年度～2017年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"15K11242","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清水, 香澄","creatorNameLang":"ja"},{"creatorName":"シミズ, カスミ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIMIZU, Kasumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"43307","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村田, 琢","creatorNameLang":"ja"},{"creatorName":"ムラタ, タク","creatorNameLang":"ja-Kana"},{"creatorName":"MURATA, Taku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"43308","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-22"}],"displaytype":"detail","filename":"2019RP0063.pdf","filesize":[{"value":"210.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2019RP0063","url":"https://mie-u.repo.nii.ac.jp/record/13287/files/2019RP0063.pdf"},"version_id":"ce76d372-da3f-40c4-a23c-1af2fcef4823"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"悪性腫瘍","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"PDE1阻害剤による口腔悪性黒色腫の転移抑制機構の解明と新規治療法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PDE1阻害剤による口腔悪性黒色腫の転移抑制機構の解明と新規治療法の開発","subitem_title_language":"ja"},{"subitem_title":"Elucidation of metastasis regulation mechanism of oral malignant melanoma by phosphodiesterase 1 inhibitor for novel therapeutic method","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692684750464"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-22"},"publish_date":"2020-01-22","publish_status":"0","recid":"13287","relation_version_is_last":true,"title":["PDE1阻害剤による口腔悪性黒色腫の転移抑制機構の解明と新規治療法の開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-14T00:30:20.321474+00:00"}