{"created":"2023-06-19T11:43:40.537752+00:00","id":13308,"links":{},"metadata":{"_buckets":{"deposit":"b98f5dfa-e3ae-4027-b140-18968feae912"},"_deposit":{"created_by":15,"id":"13308","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"13308"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00013308","sets":["420:724:1692755176241"]},"author_link":["43424"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04-13","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ゼブラフィッシュで機能的なCRISPR-Cas9システム用プラスミドの構築を目指した。すなわち、当研究室で開発したゼブラフィッシュ全身発現用プロモーター zef1alphaを用いてCas9タンパク質を発現し、一方でゼブラフィッシュ由来U6プロモーターを用いてguide RNA (gRNA)の発現を行った。構築したプラスミドをインジェクションした24hpf胚において、Cas9遺伝子の下流に位置する蛍光タンパク質（EGFP）の発現を確認するとともに、Cas9タンパク質の発現も確認した。一方、U6プロモーターによってビトロジェニン1(Vtg1)遺伝子で設計したgRNAの発現にも成功した。","subitem_description_type":"Abstract"},{"subitem_description":"We attempted to construct a functional plasmid for CRISPR-Cas9 system in zebrafish. While the EF-1 alpha (zef1alpha) promoter from zebrafish developed by our laboratory was used for Cas9 protein expression, the U6 promoter newly cloned from zebrafish was used for the expression of guide RNA (gRNA). After an injection to fertilized eggs from zebrafish with the constructed plasmid, both EGFP expression downstream of Cas9 gene in the plasmid and Cas9 protein expression were observed among zebrafish 24 hours-per-fertilization (hpf) embryos. Furthermore, gRNAs designed by the vitellogenin gene in zebrafish genome were successfully expressed.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2016年度～2017年度科学研究費補助金(挑戦的萌芽研究)研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"16K14984","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田丸, 浩","creatorNameLang":"ja"},{"creatorName":"タマル, ユヤカ","creatorNameLang":"ja-Kana"},{"creatorName":"Tamaru, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"43424","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-22"}],"displaytype":"detail","filename":"2019RP0095.pdf","filesize":[{"value":"448.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2019RP0095","url":"https://mie-u.repo.nii.ac.jp/record/13308/files/2019RP0095.pdf"},"version_id":"4e24511a-eb81-4d5e-bb9a-3af75dea2d3b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ゲノム編集技術","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼブラフィッシュ","subitem_subject_scheme":"Other"},{"subitem_subject":"Crispr-Cas9","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"魚卵をターゲットとした超効率・超集積型タンパク質発現系の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"魚卵をターゲットとした超効率・超集積型タンパク質発現系の開発","subitem_title_language":"ja"},{"subitem_title":"Development of highly efficient and accumulated protein expression system targeted to fish eggs","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692755176241"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-22"},"publish_date":"2020-01-22","publish_status":"0","recid":"13308","relation_version_is_last":true,"title":["魚卵をターゲットとした超効率・超集積型タンパク質発現系の開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-21T00:57:09.140940+00:00"}