{"created":"2023-06-19T11:44:03.202835+00:00","id":13835,"links":{},"metadata":{"_buckets":{"deposit":"92ea5886-8efd-4944-8f3a-83b41a0307fb"},"_deposit":{"created_by":15,"id":"13835","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"13835"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00013835","sets":["334:627:1692670462461"]},"author_link":["45147","45148","45149"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-05-20","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、肥大型心筋症の病態に関連する新たな遺伝子を同定することを目的として、5種類の異なる原因により発症した肥大型心筋症の網羅的遺伝子発現データを公共データベースから入手し、比較トランスクリプトーム解析を行った。その結果、5種類の肥大型心筋症モデルに共通して、酸化ストレスを制御するGSTK1の発現が低下していることを見出した。ゲノム編集技術を用いてgstk1ノックアウトゼブラフィッシュを作製し、心機能を解析したところ、肥大型心筋症に類似する病態が認められたことから、GSTK1の発現低下は肥大型心筋症の発症と密接に関連する可能性が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"In this study, we performed comparative transcriptome analysis to identify dysregulated genes common to five mouse hypertrophic cardiomyopathy(HCM) models of differing etiology. Gene-by-gene comparison identified five genes dysregukated in all five HCM models.Glutathione S-transdferase kappa 1 (Gstk1) was significantly downregulated in the five models. It has been known that GSTK1 is related to  oxidative stress. The association between HCM and GSTK1, however, has not been reported. To investigate the implications of GSTK1 downregulation for cardiac function, we knocked out gstk1 in zebrafish using CRISPR/Cas9 system. In vivo imaging of zebrafish expressing a fluorescent protein in cardiomyocytes showed that gstk1 deletion significantly decreased the end diastolic volume and, to a lesser extent, end systolic volume. These results suggest that downregulation of GSTK1 may be a common mechanism underlying HCM of various etiologies, possibly through increasing oxidative stress.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2016年度～2018年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"16K08547","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西村, 有平","creatorNameLang":"ja"},{"creatorName":"ニシムラ, ユウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishimura, Yuhei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 利男","creatorNameLang":"ja"},{"creatorName":"タナカ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"川瀬, 玲子","creatorNameLang":"ja"},{"creatorName":"カワセ, レイコ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawase, Reiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-12"}],"displaytype":"detail","filename":"2020RP0016.pdf","filesize":[{"value":"493.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2020RP0016","url":"https://mie-u.repo.nii.ac.jp/record/13835/files/2020RP0016.pdf"},"version_id":"034922fc-1134-4a09-b0d2-5ae8dec0eef9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"肥大型心筋症","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスクリプトーム","subitem_subject_scheme":"Other"},{"subitem_subject":"オミクス解析","subitem_subject_scheme":"Other"},{"subitem_subject":"データベース","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼブラフィッシュ","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノム編集","subitem_subject_scheme":"Other"},{"subitem_subject":"酸化ストレス","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新しい肥大型心筋症病態遺伝子のシステムズ薬理学","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新しい肥大型心筋症病態遺伝子のシステムズ薬理学","subitem_title_language":"ja"},{"subitem_title":"Systems pharmacology for discovering novel genes related to hypertrophic cardiomyopathy","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670462461"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-11-12"},"publish_date":"2020-11-12","publish_status":"0","recid":"13835","relation_version_is_last":true,"title":["新しい肥大型心筋症病態遺伝子のシステムズ薬理学"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-09T02:49:32.681931+00:00"}