@techreport{oai:mie-u.repo.nii.ac.jp:00013853,
 author = {山崎, 英俊 and TAMAZAKI, HIDETOSHI},
 month = {Jun},
 note = {application/pdf, 我々は、マウス胚性幹細胞株から象牙芽細胞特異的遺伝子Dsppやエナメル芽細胞特異的遺伝子Amelxの発現を誘導できる培養系を確立した。培養系で象牙芽細胞やエナメル芽細胞が誘導されているかを明らかにするために象牙芽細胞を緑色蛍光、エナメル芽細胞を赤色蛍光で検出できるマウスを作成し、象牙芽細胞及びエナメル芽細胞が蛍光標識できることを確認した。エナメル芽細胞及び象牙芽細胞を蛍光検出できる胚性幹細胞株を用いて試験管内で象牙芽細胞とエナメル芽細胞の誘導を試みたが、同定には至っていない。胚性幹細胞株と正常マウス歯上皮や歯間葉細胞を混合し、歯胚形成における胚性幹細胞由来の蛍光細胞の歯への寄与を検討している。, By using mouse embryonic stem cells, we found that cells from ES cell culture expressed Dspp or Amelx. To investigate if Dspp-expressing odontoblasts or Amelx-expressing ameloblasts were induced from ESCs in this culture, we had established Amelx-TdTomato Knock In (Amelx TdTomato/+) mice and Dspp-GFP knock In (Dspp GFP/+) mice to identify ameloblasts and odontoblasts as TdTomato-expressing cells, and GFP-expressing cells, respectively. Using these mice, we found that TdTomato and GFP were strongly expressed on ameloblasts and odontoblasts, respectively. Furthermore, we had established ES cell lines from these transgenic mice. Although we tried to induce GFP-expressing odontoblast and TdTomato-expressing ameloblasts from ESC cultures, we had not identified odontoblasts and ameloblasts from ESCs. Using cells from embryonic stem cells with dental epithelium and dental mesenchyme of wild-type embryos and try to examine the contribution of cells from ESCs to tooth formation., 2016年度～2018年度科学研究費補助金(挑戦的萌芽研究)研究成果報告書, 16K15774},
 title = {胚性幹細胞を用いた歯の器官形成過程の解析及び歯の器官形成の試み},
 year = {2019},
 yomi = {ヤマザキ, ヒデトシ}
}