{"created":"2023-06-19T11:44:23.830347+00:00","id":14307,"links":{},"metadata":{"_buckets":{"deposit":"c64ad53f-7ff4-4e57-a928-3d5f264b35c8"},"_deposit":{"created_by":16,"id":"14307","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"14307"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014307","sets":["334:608:618:1262"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09-16","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-09-16"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Study Design. Biochemical and immunohistochemical analyses by the human intervertebral disc (IVD) cells and tissues.\nObjective. To examine the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptors, GDNF family receptor (GFR) al and rearranged during transfection (RET) in the human IVD cells and the tissues with the early and advanced stages of degeneration.\nSummary of Background Data. The neurotrophin family, including nerve growth factor (NGF), has been reported to be expressed in the IVDs and plays a role in hyperalgesia and neuronal sensitization. Despite having properties similar to the NGF, the expression of GDNF in the IVD remains unknown.\nMethods. Human IVD cells were cultured in monolayer. Immunohistochemical analyses and western blotting were performed to examine the protein levels of GDNF and its receptors. To examine the effect of proinflammatory cytokines, cells were cultured in the presence of interleukin-1β(IL-1β). The immunohistochemical expression of these proteins was also evaluated using human IVD tissues with different stages of degeneration.\nResults. Immunofluorescent reactivity against anti-GDNF, GFRα1, and RET antibodies was identified in human IVD cells. In protein extracts from IVD cells, those protein expressions were also identified by Western blot. IL-1β significantly stimulated the mRNA expression of GDNF compared to that of the control group. There was no significant effect of IL-1β on the mRNA expression of GFRα1 and RET. The percentage of GDNF-immunopositive cells in advanced degenerated discs was significantly higher than that in early degenerated discs, whereas those of GFRα1 and RET showed no significant differences.\nConclusions. GDNF and its receptors were constitutively expressed in the human IVD cells. GDNF expression was significantly enhanced by proinflammatory stimuli, and in the microenvironment with advanced tissue degeneration.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Orthopaedic Surgery, Mie University Graduate School of Medicine","subitem_description_type":"Other"},{"subitem_description":"20p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙医学第1055号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1097/BRS.0000000000003418","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Spine.45(13):E768-E775に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamada, Junichi","creatorNameLang":"en"},{"creatorName":"ヤマダ, ジュンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"山田, 淳一","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-06"}],"displaytype":"detail","filename":"2020DM0918.pdf","filesize":[{"value":"6.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2020DM0918","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/14307/files/2020DM0918.pdf"},"version_id":"763f00b2-61f1-4ea6-b12c-9ceb116ef57c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"neurotrophin family","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glial cell line-derived neurotrophic factor (GDNF)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intervertebral disc","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Low back pain","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Expression of glial cell line-derived neurotrophic factor in the human intervertebral disc","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of glial cell line-derived neurotrophic factor in the human intervertebral disc","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1262"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-10-06"},"publish_date":"2021-10-06","publish_status":"0","recid":"14307","relation_version_is_last":true,"title":["Expression of glial cell line-derived neurotrophic factor in the human intervertebral disc"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-25T04:19:35.620367+00:00"}