{"created":"2023-06-19T11:44:27.891533+00:00","id":14399,"links":{},"metadata":{"_buckets":{"deposit":"f2f0d0ff-be6d-474e-9ff0-f82e3e9ecc1f"},"_deposit":{"created_by":16,"id":"14399","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"14399"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014399","sets":["334:608:618:1308"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-21","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-07-21"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Branched-chain amino acids (BCAA) reverse malnutrition and L-carnitine leads to the reduction of hyperammonemia and muscle cramps in cirrhotic patients. BCAA and L-carnitine are involved in glucose and fatty acid metabolism, however their mechanistic activity in cirrhotic liver is not fully understood. We aim to define the molecular mechanism(s) and combined effects of BCAA and L-carnitine using a cirrhotic rat model. Rats were administered carbon tetrachloride for 10 weeks to induce cirrhosis. During the last 6 weeks of administration, cirrhotic rats received BCAA, L-carnitine or a combination of BCAA and L-carnitine daily via gavage. We found that BCAA and L-carnitine treatments significantly improved hepatocellular function associated with reduced triglyceride level, lipid deposition and adipophilin expression, in cirrhotic liver. Lipidomic analysis revealed dynamic changes in hepatic lipid composition by BCAA and L-carnitine administrations. BCAA and L-carnitine globally increased molecular species of phosphatidylcholine. Liver triacylglycerol and phosphatidylcholine hydroperoxides were significantly decreased by BCAA and L-carnitine. Furthermore, serum and liver ATP levels were significantly increased in all treatments, which were attributed to the elevation of mature cardiolipins and mitochondrial component gene expressions. Finally, BCAA and L-carnitine dramatically reduced hepatocellular death. In conclusion, BCAA and L-carnitine treatments attenuate hepatocellular damage through the reduction of lipid peroxides and the overall maintenance of mitochondrial integrity within the cirrhotic liver. These effectiveness of BCAA and L-carnitine support the therapeutic strategies in human chronic liver diseases.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Gastroenterology and Hepatology, Graduate School of Medicine, Mie University, Tsu, Japan","subitem_description_type":"Other"},{"subitem_description":"11p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2080号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.biopha.2020.111181","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Biomedicine & Pharmacotherapy 135 (2021) に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tamai, Yasuyuki","creatorNameLang":"en"},{"creatorName":"タマイ, ヤスユキ","creatorNameLang":"ja-Kana"},{"creatorName":"玉井, 康将","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-16"}],"displaytype":"detail","filename":"2021DM0704.pdf","filesize":[{"value":"6.9 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2021DM0704","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/14399/files/2021DM0704.pdf"},"version_id":"c743ec37-5469-48fc-8142-768f5be5f941"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"L-carnitine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Branched-chain amino acids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cirrhosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipid peroxides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cardiolipins","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Branched-chain amino acids and L-carnitine attenuate lipotoxic hepatocellular damage in rat cirrhotic liver","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Branched-chain amino acids and L-carnitine attenuate lipotoxic hepatocellular damage in rat cirrhotic liver","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1308"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-16"},"publish_date":"2021-11-16","publish_status":"0","recid":"14399","relation_version_is_last":true,"title":["Branched-chain amino acids and L-carnitine attenuate lipotoxic hepatocellular damage in rat cirrhotic liver"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-17T02:03:07.508057+00:00"}