{"created":"2023-06-19T11:44:30.412281+00:00","id":14449,"links":{},"metadata":{"_buckets":{"deposit":"adfb2897-1c92-4474-9afd-2f38fb0c595b"},"_deposit":{"created_by":16,"id":"14449","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"14449"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014449","sets":["334:608:618:1308"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-15","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-09-15"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Phenotypic screening is one of the most practical approaches to the identification of mediators of behaviour, since it is difficult to model brain function in vitro, at a cellular level. We used a zebrafish (Danio rerio) behavioural assay to discover novel, natural, neuroactive compounds.\nMaterials and methods: A zebrafish behavioural assay was performed for seven natural compounds, obtained from plants. The behavioural profiles were compared to those of known psychoactive drugs. We characterised a natural compound exhibiting a behaviour profile similar to that of suvorexant, using in silico, in vitro and microarray expression analysis.\nResults: The behavioural analysis performed in this study classified central nervous system drugs according to their mechanism. Zebrafish treated with a natural compound, 8β-(4'-Hydroxytigloyloxy) costunolide (8β), showed behaviour profiles similar to those of zebrafish treated with suvorexant, a known orexin antagonist. This behavioural assay was validated using in silico and in vitro assays, which revealed that the new compound was a dual orexin receptor antagonist. In addition, transcriptome analysis suggested that 8β might regulate the nuclear factor-κB (NF-κB) related pathway.\nConclusions: We conclude that zebrafish phenotypic screening, combined with in silico assays and gene expression profiling, is a useful strategy to discover and characterize novel therapeutic compounds, including natural products.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Systems Pharmacology, Mie University, Graduate School of Medicine, Tsu, Mie, Japan","subitem_description_type":"Other"},{"subitem_description":"13p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2082号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.26355/eurrev_202005_21207   \n ","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"European Review for Medical and Pharmacological Sciences,24 (9): 5127-5139に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMANAKA, MIKIKO","creatorNameLang":"en"},{"creatorName":"ヤマナカ, ミキコ","creatorNameLang":"ja-Kana"},{"creatorName":"山中, 実喜子","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-04"}],"displaytype":"detail","filename":"2021DM0902.pdf","filesize":[{"value":"6.5 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2021DM0902","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/14449/files/2021DM0902.pdf"},"version_id":"5cbd48ef-cc70-4688-8066-7021b91ce578"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Zebrafish","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Behaviour","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phenotype-based screening","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sleep","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Orexin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1308"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-01-04"},"publish_date":"2022-01-04","publish_status":"0","recid":"14449","relation_version_is_last":true,"title":["A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-17T02:03:19.055103+00:00"}