@techreport{oai:mie-u.repo.nii.ac.jp:00014499,
 author = {吉山, 繁幸 and Yoshiyama, Shigeyuki and 問山, 裕二 and Toiyama, Yuji and 楠, 正人 and Kusunoki, Masato and 安田, 裕美 and Yasuda, Hiromi and 毛利, 靖彦 and Mohri, Yasuhiko},
 month = {May},
 note = {application/pdf, 胃癌細胞株ならびに胃間質細胞株の培養上清液より、別記の方法でエクソソームRNAを抽出しmicroRNA arrayに外注依頼し、データ解析し胃癌細胞株で異常発現している12miRNAを候補miRNAとして選定した。Preliminay studyとしれ胃癌患者(n=10)ならびに健常者(n=10)の血清よりエクソソームRNAを抽出しqRT-PCR法で候補miRNAを定量し、統計解析ソフトでリスクスコアを作成した所、胃癌患者では健常人と比べてリスクスコアが有意に高値であり(P<0.0001)、リスクスコアによってArea Under Curve(AUC):0.966の精度で胃癌患者を識別しえた。, We extracted exosomal-RNA from the culture-supernatant of gastric cancer cell line and gastric stromal cell line by the method mentioned separately, offered microRNA-array to outsource, then analyzed the data and identified 12 miRNA which were significantly dysregulated in gastric cancer cell line. As a preliminay study, exosomal RNA was extracted from the sera of gastric cancer patients (n=10) and healthy subjects(n=10). Then the candidate miRNA were quantified by qRT-PCR method, and risk score was created by statistical software. The risk score was significantly higher in gastric cancer patients than that in healthy controls (p<0.0001), and discriminated gastric cancer patients by the accuracy pf Area Under Curve (AUC): 0.966., 2017年度～2019年度科学研究費補助金(基盤研究(C))研究成果報告書, 17K10583},
 title = {エクソソームに包埋された核酸を用いた胃癌早期診断法の確立},
 year = {2020},
 yomi = {ヨシヤマ, シゲユキ and トイヤマ, ユウジ and クスノキ, マサト and ヤスダ, ヒロミ and モウリ, ヤスヒコ}
}