{"created":"2023-06-19T11:44:32.618635+00:00","id":14499,"links":{},"metadata":{"_buckets":{"deposit":"0364054c-1ebb-4ab3-9aa2-2e26e39e704d"},"_deposit":{"created_by":15,"id":"14499","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"14499"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014499","sets":["334:627:1692670486782"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-18","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"胃癌細胞株ならびに胃間質細胞株の培養上清液より、別記の方法でエクソソームRNAを抽出しmicroRNA arrayに外注依頼し、データ解析し胃癌細胞株で異常発現している12miRNAを候補miRNAとして選定した。Preliminay studyとしれ胃癌患者(n=10)ならびに健常者(n=10)の血清よりエクソソームRNAを抽出しqRT-PCR法で候補miRNAを定量し、統計解析ソフトでリスクスコアを作成した所、胃癌患者では健常人と比べてリスクスコアが有意に高値であり(P<0.0001)、リスクスコアによってArea Under Curve(AUC):0.966の精度で胃癌患者を識別しえた。","subitem_description_type":"Abstract"},{"subitem_description":" We extracted exosomal-RNA from the culture-supernatant of gastric cancer cell line and gastric stromal cell line by the method mentioned separately, offered microRNA-array to outsource, then analyzed the data and identified 12 miRNA which were significantly dysregulated in gastric cancer cell line. As a preliminay study, exosomal RNA was extracted from the sera of gastric cancer patients (n=10) and healthy subjects(n=10). Then the candidate miRNA were quantified by qRT-PCR method, and risk score was created by statistical software. The risk score was significantly higher in gastric cancer patients than that in healthy controls (p<0.0001), and discriminated gastric cancer patients by the accuracy pf Area Under Curve (AUC): 0.966.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2017年度～2019年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"17K10583","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉山, 繁幸","creatorNameLang":"ja"},{"creatorName":"ヨシヤマ, シゲユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshiyama, Shigeyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"問山, 裕二","creatorNameLang":"ja"},{"creatorName":"トイヤマ, ユウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Toiyama, Yuji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"楠, 正人","creatorNameLang":"ja"},{"creatorName":"クスノキ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"Kusunoki, Masato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"安田, 裕美","creatorNameLang":"ja"},{"creatorName":"ヤスダ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"Yasuda, Hiromi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"毛利, 靖彦","creatorNameLang":"ja"},{"creatorName":"モウリ, ヤスヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Mohri, Yasuhiko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-19"}],"displaytype":"detail","filename":"2021RP0033.pdf","filesize":[{"value":"193.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2021RP0033","url":"https://mie-u.repo.nii.ac.jp/record/14499/files/2021RP0033.pdf"},"version_id":"bed77a13-cfe9-4ed6-aef8-2a2e330d1d22"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"胃癌","subitem_subject_scheme":"Other"},{"subitem_subject":"バイオマーカー","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"エクソソームに包埋された核酸を用いた胃癌早期診断法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エクソソームに包埋された核酸を用いた胃癌早期診断法の確立","subitem_title_language":"ja"},{"subitem_title":"establishment of early diagnostic method in gastric cancer using exosome RNA","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670486782"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-01-19"},"publish_date":"2022-01-19","publish_status":"0","recid":"14499","relation_version_is_last":true,"title":["エクソソームに包埋された核酸を用いた胃癌早期診断法の確立"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-09T05:32:19.903599+00:00"}