{"created":"2023-06-19T11:44:33.040417+00:00","id":14508,"links":{},"metadata":{"_buckets":{"deposit":"cfdc09d2-d819-41e9-b59d-646e6593a1db"},"_deposit":{"created_by":15,"id":"14508","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"14508"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014508","sets":["334:627:1692670486782"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-19","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"天然に近い状態の膜タンパク質の立体構造を観察することは、その生理的機能を明らかにするうえで重要な知見が得られる可能性が高い。このような構造解析としては、脂質二重膜中に再構成された膜タンパク質がシート状に整列する二次元結晶を用いた電子線結晶解析が適しているが、たとえ結晶作製に成功しても分解能が悪いことも多く電子線結晶解析に適用できない場合も多々ある。本研究では、このような従来の解析方法では歯がたたなかった結晶性のよくない二次元結晶でも、構造解析が行えるように、電子線トモグラフィーと結晶学を組み合わせた新規手法の確率ならびに評価を行った。","subitem_description_type":"Abstract"},{"subitem_description":" Observing the three-dimensional structure of a membrane protein embeded into lipids, close to natural environment, is highly lilely to provide important knowledge for clarifying its physiological function. For such structural analysis, electron crystallograpy using a two-dimensional (2D) crystal of membrane protein is definitely suitable. Under some conditions, membrane proteins are arranged in a 2D sheet after   reconstitution into a lipid bilayer. However even if the 2D crystal production is successful, the possibility of obtaining a crytal giving good diffraction is quite low. Most of them cannot be applied to electron crystallography. In this study, we established and evaluated a method that enables structural analysis of 2D crystals with poor crystallinity that were not tackled by conventional analysis methods.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2017年度～2019年度科学研究費補助金(挑戦的萌芽研究)研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"17K19347","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷, 一寿","creatorNameLang":"ja"},{"creatorName":"タニ, カズトシ","creatorNameLang":"ja-Kana"},{"creatorName":"Tani, Kazutoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-19"}],"displaytype":"detail","filename":"2021RP0042.pdf","filesize":[{"value":"496.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2021RP0042","url":"https://mie-u.repo.nii.ac.jp/record/14508/files/2021RP0042.pdf"},"version_id":"4be0646a-cf85-4644-94e2-5442895e161a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クライオ電子顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"電子線結晶学","subitem_subject_scheme":"Other"},{"subitem_subject":"クライオトモグラフィー","subitem_subject_scheme":"Other"},{"subitem_subject":"二次元結晶","subitem_subject_scheme":"Other"},{"subitem_subject":"F型ATPase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"電子線トモグラフィーと結晶学を組み合わせた膜タンパク質の構造解析手法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"電子線トモグラフィーと結晶学を組み合わせた膜タンパク質の構造解析手法の確立","subitem_title_language":"ja"},{"subitem_title":"Electron crystallographic analysis of tomographic volumes of 2D crystals of membrane proteins","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670486782"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-01-19"},"publish_date":"2022-01-19","publish_status":"0","recid":"14508","relation_version_is_last":true,"title":["電子線トモグラフィーと結晶学を組み合わせた膜タンパク質の構造解析手法の確立"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-09T05:32:43.482398+00:00"}