@techreport{oai:mie-u.repo.nii.ac.jp:00014543,
 author = {島本, 亮 and Shimamoto, Akira},
 month = {Jun},
 note = {【目的】心停止後臓器提供を可能にすべく、ドナー肺長時間保存法を確立する。特に、heat shock proteins(HSPs)を用いた長時間作動のpharmacological preconditioning (PPC)について模索する。【方法】HSP-27を前投与したマウスに肺虚血再灌流を負荷し肺障害及び細胞内シグナル活性を測定した。【結果】HSP-27投与で肺虚血再灌流障害は有意に抑制されたが、NF-κBの有意な活性化を認めなかった。【考察】HSP-27を用いたPPCが確立された。NF-κBの関与を必要とせずHSP-27の直接的作用か、間接的なapoptosis抑制作用かが示唆された。, Purpose: We hypothesized that exogenous heat shock proteins (HSPs) would provide pharmacological preconditioning (PPC). In the present study, we examined whether phosphorylated recombinant HSP27 (prHSP27), which recombinant HSP27 phosphorylated by MAPKAP kinase 2 in vitro, affected PPC to protect against lung ischemia-reperfusion (I/R) injury (LIRI). Methods: C57BL/6 mice received prHSP27(2.5 g/kg) or vehicle 30 minutes prior to 60 minutes of ischemia of their left lungs, followed by 180 minutes of reperfusion or ischemic preconditioning (IPC) with six cycles of 5 minutes ischemia and 5 minutes reperfusion prior to I/R. Results: Pretreatment of mice with prHSP27 resulted in the development of a significant smaller LIRI when compared with vehicle treated lungs. Conclusions: In this study, we demonstrated that prHSP27 migrates into lung tissue and maintains its activation. It suggests that prHSP27 may be a useful therapeutic agent to protect against LIRI as PPC., 2016年度～2019年度科学研究費補助金(基盤研究(C))研究成果報告書, 16K10676},
 title = {肺移植におけるドナー肺長時間保存法の確立ーより長時間作動PPCの模索ー},
 year = {2020},
 yomi = {シマモト, アキラ}
}