{"created":"2023-06-19T11:44:35.161089+00:00","id":14557,"links":{},"metadata":{"_buckets":{"deposit":"1e66f178-f9a2-4e71-8b2b-4cfa446a8d5d"},"_deposit":{"created_by":15,"id":"14557","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"14557"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014557","sets":["366:680:1692685146800"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-03","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"細胞情報や細胞骨格に関連する膜タンパク質受容体などを、細胞膜を模倣して作製した人工細胞膜へ再構成する手法を開発し、さらに、例えば、細胞動態のモデリングに繋げるための解析実験法提案することを、めざして行った。顕微鏡で直接観察が可能である界面通過法（張り合わせ法）で作製した巨大ベシクルGUVへGPCR等組換え膜タンパク質をバキュロウイルス出芽粒子（budded virions, BVs）の膜融合で取り込ませた。細胞様ミクロコ ンパートメントを狙い、新規にGUV大量調製法を考案し、また細胞骨格タンパク質を効率的に封入するためのミクロな水性分離技術も研究した。以上により人工細胞システムの改良を図った。","subitem_description_type":"Abstract"},{"subitem_description":"In this project, we tried to study and develop how to incorporate proteins related to cell signal transduction and cell motility into artificial lipid bilayer systems mimicking actual membranes of living cells. In addition, through realization of such model systems, we would like to believe we can invent a novel analytical protocol for artificial cellular dynamics. In this study, we demonstrated the following results: reconstitution of recombinant membrane proteins like GPCRs into giant unilamellar vesicles (GUVs) through membrane fusion with baculovirus budded virions (BVs), high-yield preparation of GUVs with high density, and apparently selective accumulation of the biomacromolecules cytoskeletal proteins like filamentous actin into aqueous microcompartments.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2016年度～2019年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"16K07293","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"湊元, 幹太","creatorNameLang":"ja"},{"creatorName":"ツモト, カンタ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsumoto, Kanta","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"瀧口, 金吾","creatorNameLang":"ja"},{"creatorName":"タキグチ, キンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"Takiguchi, Kingo","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-24"}],"displaytype":"detail","filename":"2021RP0091.pdf","filesize":[{"value":"449.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2021RP0091","url":"https://mie-u.repo.nii.ac.jp/record/14557/files/2021RP0091.pdf"},"version_id":"1e5d0d5f-4313-40bc-a1c7-34da38058843"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"人工細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞情報","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオリポソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"膜タンパク質","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞骨格","subitem_subject_scheme":"Other"},{"subitem_subject":"脂質二分子膜","subitem_subject_scheme":"Other"},{"subitem_subject":"GUV","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロコンパートメント","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"人工細胞システムによる細胞情報クロストークの実現と細胞動態解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"人工細胞システムによる細胞情報クロストークの実現と細胞動態解析","subitem_title_language":"ja"},{"subitem_title":"Artificial cell systems for modeling of cell-information cross-talk and cell dynamic analysis","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692685146800"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-01-24"},"publish_date":"2022-01-24","publish_status":"0","recid":"14557","relation_version_is_last":true,"title":["人工細胞システムによる細胞情報クロストークの実現と細胞動態解析"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-20T01:44:02.206920+00:00"}