{"created":"2023-06-19T11:44:50.187750+00:00","id":14965,"links":{},"metadata":{"_buckets":{"deposit":"82ad5ab8-6909-47bc-a10e-c4ee56ab8abb"},"_deposit":{"created_by":16,"id":"14965","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"14965"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00014965","sets":["334:608:618:1308"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Establishing an optimal animal model for intervertebral disc (IVD) degeneration is essential for developing new IVD therapies. The intra-articular injection of monosodium iodoacetate (MIA), which is commonly used in animal models of osteoarthritis, induces cartilage degeneration and progressive arthritis in a dose- and timedependent manner. The purpose of this study was to determine the effect of MIA injections into rabbit IVDs on the progression of IVD degeneration evaluated by radiographic, micro-computerized tomography (micro-CT), magnetic resonance imaging (MRI), and histological analyses.\nMethods: In total, 24 New Zealand White (NZW) rabbits were used in this study. Under general anesthesia, lumbar discs from L1–L2 to L4–L5 had a posterolateral percutaneous injection of MIA in contrast agent (CA) (L1–L2: CA only; L2–L3: MIA 0.01 mg; L3–L4: 0.1 mg; L4–L5: 1.0 mg; L5–L6: non-injection (NI) control). Disc height was radiographically monitored biweekly until 12 weeks after injection. Six rabbits were sacrificed at 2, 4, 8, and 12 weeks post-injection and processed for micro-CT, MRI (T2-mapping), and histological analyses. Three-dimensional (3D) disc height in five anatomical zones was evaluated by 3D reconstruction of micro-CT data.\nResults: Disc height of MIA-injected discs (L2–L3 to L4–L5) gradually decreased time-dependently (P < 0.0001). The disc height of MIA 0.01 mg-injected discs was significantly higher than those of MIA 0.1 and 1.0 mg-injected discs (P< 0.01, respectively). 3D micro-CT analysis showed the dose- and time-dependent decrease of 3D disc height of MIAinjected discs predominantly in the posterior annulus fibrosus (AF) zone. MRI T2 values of MIA 0.1 and 1.0 mg-injected discs were significantly decreased compared to those of CA and/or NI controls (P < 0.05). Histological analyses showed progressive time- and dose-degenerative changes in the discs injected with MIA (P < 0.01). MIA induced cell death in the rabbit nucleus pulposus with a high percentage, while the percentage of cell clones was low.\nConclusions: The results of this study showed, for the first time, that the intradiscal injection of MIA induced degenerative changes of rabbit IVDs in a time-and dose-dependent manner. This study suggests that MIA injection into rabbit IVDs could be used as an animal model of IVD degeneration for developing future treatments.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, 2‑174 Edobashi, Tsu City, Mie 514‑8507, Japan","subitem_description_type":"Other"},{"subitem_description":"13p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2117号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s13075-021-02686-6","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Arthritis Research & Therapy (2021) 23:297に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sudo, Takao","creatorNameLang":"en"},{"creatorName":"スドウ, タカオ","creatorNameLang":"ja-Kana"},{"creatorName":"須藤, 隆夫","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-20"}],"displaytype":"detail","filename":"2021DM0330.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2021DM0330","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/14965/files/2021DM0330.pdf"},"version_id":"09c2f247-26dc-4b48-bce2-6cff1c3421f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Intervertebral disc","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Disc degeneration","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Monosodium iodoacetate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"μCT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MRI","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Histology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rabbit","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animal model","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Intradiscal injection of monosodium iodoacetate induces intervertebral disc degeneration in an experimental rabbit model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intradiscal injection of monosodium iodoacetate induces intervertebral disc degeneration in an experimental rabbit model","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1308"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-20"},"publish_date":"2022-07-20","publish_status":"0","recid":"14965","relation_version_is_last":true,"title":["Intradiscal injection of monosodium iodoacetate induces intervertebral disc degeneration in an experimental rabbit model"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-17T02:04:32.130176+00:00"}