{"created":"2023-06-19T11:45:04.049355+00:00","id":15278,"links":{},"metadata":{"_buckets":{"deposit":"3cd3dedb-d55d-4922-ac2d-d0204dc4c72d"},"_deposit":{"created_by":15,"id":"15278","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"15278"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00015278","sets":["334:627:1692670514969"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-16","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト凍結脳組織から、質量分析（MS）解析に使用するためのタウタンパク質の精製手法を確立した。さらに特殊なMS解析を用いて、健常者およびアルツハイマー病患者脳におけるタウタンパク質のリン酸化状態を解析した。予測解析の結果から、181個のリン酸化アミノ酸配列を同定した。さらに同定されたペプチド（アミノ酸を構成するより小さい単位）のリストについて、健常者とアルツハイマー病患者脳との間で、各ペプチド毎にリン酸化状態の明確な差異を検出するに至った。","subitem_description_type":"Abstract"},{"subitem_description":"We have established a method for purifying tau protein from human frozen brain tissue for use in mass spectrometry (MS) analysis. In addition, a specific MS analysis was used to analyze the phosphorylation status of tau protein in the brains of healthy subjects and Alzheimer's disease patients. From the results of predictive analysis, 181 phosphorylated amino acid sequences were identified. For a list of further identified peptides (smaller units that make up amino acids), a clear difference in phosphorylation status was detected for each peptide between healthy subjects and the brains of Alzheimer's disease patients.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2020年度科学研究費補助金(若手研究)研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K17002","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森本, 悟","creatorNameLang":"ja"},{"creatorName":"モリモト, サトル","creatorNameLang":"ja-Kana"},{"creatorName":"Morimoto, Satoru","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-24"}],"displaytype":"detail","filename":"2022RP0039.pdf","filesize":[{"value":"404.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2022RP0039","url":"https://mie-u.repo.nii.ac.jp/record/15278/files/2022RP0039.pdf"},"version_id":"c2ddadbb-2619-4803-9b33-9b92d480ad6d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Kii ALS/PDC","subitem_subject_scheme":"Other"},{"subitem_subject":"tau","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"mass spectrometry","subitem_subject_scheme":"Other"},{"subitem_subject":"pluripotent stem cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"多能性幹細胞とヒト凍結脳のタウ蛋白における網羅的リン酸化比較解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"多能性幹細胞とヒト凍結脳のタウ蛋白における網羅的リン酸化比較解析","subitem_title_language":"ja"},{"subitem_title":"Comprehensive analysis of tau protein phosphorylation in pluripotent stem cells and human brain","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692670514969"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-24"},"publish_date":"2022-11-24","publish_status":"0","recid":"15278","relation_version_is_last":true,"title":["多能性幹細胞とヒト凍結脳のタウ蛋白における網羅的リン酸化比較解析"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-10T02:02:49.721690+00:00"}