{"created":"2023-06-19T11:45:05.273972+00:00","id":15306,"links":{},"metadata":{"_buckets":{"deposit":"c9e3e844-0418-498b-9a00-7509ee853f03"},"_deposit":{"created_by":15,"id":"15306","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"15306"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00015306","sets":["366:680:1692685258258"]},"author_link":["50676","50677","50678","50679"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-18","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1)立体構造特異的ターゲティング (SST) 法を用いて、膜タンパク質に対する立体構造認識モノクローナル抗体の作製に成功した。(2)単一B細胞から、直接モノクローナル抗体(mAb)を取得するEcobody法を用いて、EGFR認識ウサギmAbおよび、Swine Influenza Virus に対するウサギmAbを取得し、そのウイルス検出系を構築した。(3)マイクロ電極を組み込んだ三次元誘電泳動デバイスを開発し、細胞のアレイ化、異種細胞ペアの形成および細胞融合を達成した。(4)マイクロビーズ担持人工脂質膜へGPCR(β2AR)等を提示する手法を組換えバキュロウイルスとの膜融合技術で実現した。","subitem_description_type":"Abstract"},{"subitem_description":"(1) Based on stereospecific targeting (SST) technique, we succeeded in producing conformation-specific monoclonal antibodies against membranous proteins with high efficiency, regardless of that it has been known to be quite difficult for generating them. (2) EGFR-recognizing and Swine Influenza Virus-binding rabbit monoclonal antibodies were obtained by using the Ecobody technology, directly from single B cells. In addition, a sand witch detection system for the virus was also constructed.(3) The formation of pairs of the different types of cells and the electrofusion of pairs were achieved by using the novel device with microband electrodes based on dielectrophoresis. (4) We developed the method for preparation of membrane protein-displaying artificial lipid membranes supported on spherical silica microbeads using direct membrane fusion with recombinant baculovirus virions expressing GPCRs like adrenergic receptors.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2017年度～2020年度科学研究費補助金(基盤研究(B))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"17H03468","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"冨田, 昌弘","creatorNameLang":"ja"},{"creatorName":"トミタ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tomita, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中野, 秀雄","creatorNameLang":"ja"},{"creatorName":"ナカノ, ヒデオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakano, Hideo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安川, 智之","creatorNameLang":"ja"},{"creatorName":"ヤスカワ, トモユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yasukawa, Tomoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"湊元, 幹太","creatorNameLang":"ja"},{"creatorName":"ツモト, カンタ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsumoto, Kanta","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-29"}],"displaytype":"detail","filename":"2022RP0067.pdf","filesize":[{"value":"656.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2022RP0067","url":"https://mie-u.repo.nii.ac.jp/record/15306/files/2022RP0067.pdf"},"version_id":"db058f61-dcd4-44c3-9eae-075f37c60966"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"立体構造認識モノクローナル抗体","subitem_subject_scheme":"Other"},{"subitem_subject":"無細胞発現系","subitem_subject_scheme":"Other"},{"subitem_subject":"誘電泳動","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオリポソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"分子標的治療薬","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"多種抗膜タンパク質抗体の高効率な一括取得法とその分子標的治療薬評価法の一体的開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"多種抗膜タンパク質抗体の高効率な一括取得法とその分子標的治療薬評価法の一体的開発","subitem_title_language":"ja"},{"subitem_title":"Simultaneous production of monoclonal antibodies against multiple membrane proteins and their evaluations for the applications to therapeutic medicines","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1692685258258"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-11-29"},"publish_date":"2022-11-29","publish_status":"0","recid":"15306","relation_version_is_last":true,"title":["多種抗膜タンパク質抗体の高効率な一括取得法とその分子標的治療薬評価法の一体的開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-20T02:11:44.461720+00:00"}