@techreport{oai:mie-u.repo.nii.ac.jp:00015319,
 author = {伊藤, 智広 and ITOH, Tomohiro},
 month = {May},
 note = {application/pdf, 骨芽細胞や破骨細胞に指令を出す骨細胞はメカニカルストレス（以下MS)を感知すると，隣接する骨芽細胞や破骨細胞に効率的に情報を伝達し，骨代謝を制御する。本研究では，この情報伝達に細胞自身が分泌する膜小胞が深く関与しているのではないかと作業仮設を立てた。MSを負荷した骨細胞から分泌した膜小胞を未分化の前駆破骨細胞に添加したところ，破骨細胞の分化が誘導された。この分化誘導機構を検討したところ，MS負荷骨細胞分泌膜小胞にしか含まれない破骨細胞分化誘導関連タンパク質の存在を確認した。今後これらタンパク質による分化誘導機構についてさらに詳細に検討することで，骨のホメオスタシス機構について明らかにしたい。, Osteocyte, which is the most abundant cell in bone tissues, is well known as a mechanical stress (MS) receiving cell. During bone remodelling, bone resorption by osteoclasts precedes bone formation by osteoblasts. However, its mechanism is still unknown. In this study, we examined whether exosome released from osteocyte by MS stimulation are involved in osteoclast differentiation.
Though the vesicles isolated from mechanical stress-loaded MLO-Y4 cells had no effect against osteoblast differentiation, these vesicles significantly induced osteoclast differentiation. To characterize the mechanisms by which mechanical stress-loaded MLO-Y4 cell vesicles induces osteoclast differentiation in murine macrophage RAW264.7 cells, we analysed vesicle membrane and vesicle internal proteins by nano-LC-MS/MS-based shotgun proteomics. As a result, Protein X , CD9 and CD63 were only detected in mechanical stress-loaded MLO-Y4 cell vesicles., 2018年度～2020年度科学研究費補助金(基盤研究(C))研究成果報告書, 18K11019},
 title = {骨代謝を制御する骨細胞が分泌する膜微小胞による破骨細胞分化誘導機構の解明},
 year = {2021},
 yomi = {イトウ, トモヒロ}
}