{"created":"2023-06-19T11:45:15.243281+00:00","id":15520,"links":{},"metadata":{"_buckets":{"deposit":"ccbbf785-644f-4b58-9636-1f2b3c41113f"},"_deposit":{"created_by":16,"id":"15520","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"15520"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00015520","sets":["334:608:618:1336"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-12-21","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-12-21"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hematopoiesis was considered a hierarchical stepwise process but was revised to a continuous process following single-cell RNA sequencing. However, the uncertainty or fluctuation of single-cell transcriptome dynamics during differentiation was not considered, and the dendritic cell (DC) pathway in the lymphoid context remains unclear. Here, we identify human B-plasmacytoid DC (pDC) bifurcation as large fluctuating transcriptome dynamics in the putative B/NK progenitor region by dry and wet methods. By converting splicing kinetics intodiffusiondynamics in a deepgenerativemodel,our original computationalmethodology reveals strongfluctuation at B/pDC bifurcation in IL-7Ra+ regions, and LFA-1 fluctuates positively in the pDCdirection at the bifurcation. These expectancies are validated by the presence of B/pDCprogenitors in the IL-7Ra+ fraction and preferential expression of LFA-1 in pDC-biased progenitors with a niche-like culture system.Weprovide a model of fluctuation-based differentiation, which reconciles continuous and discrete models and is applicable to other developmental systems.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan","subitem_description_type":"Other"},{"subitem_description":"22p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2165号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.celrep.2022.111260","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Cell Reports 40, 111260, August 30, 2022に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagaharu, Keiki","creatorNameLang":"en"},{"creatorName":"ナガハル, ケイキ","creatorNameLang":"ja-Kana"},{"creatorName":"永春, 圭規","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-04-05"}],"displaytype":"detail","filename":"2022DM1210.pdf","filesize":[{"value":"9.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2022DM1210","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/15520/files/2022DM1210.pdf"},"version_id":"dec125b6-ac13-4e9c-93dc-e6e5405a0296"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1336"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-05"},"publish_date":"2023-04-05","publish_status":"0","recid":"15520","relation_version_is_last":true,"title":["A bifurcation concept for B-lymphoid/plasmacytoid dendritic cells with largely fluctuating transcriptome dynamics"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-17T01:54:29.223130+00:00"}