{"created":"2023-06-19T11:45:15.962435+00:00","id":15531,"links":{},"metadata":{"_buckets":{"deposit":"4ad9ab59-9b54-4679-a0ab-5d1b81486f91"},"_deposit":{"created_by":16,"id":"15531","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"15531"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00015531","sets":["334:608:618:1336"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-12-21","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-12-21"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neuroblastoma is a highly malignant disease with a poor prognosis and few treatment options. Despite conventional chemotherapy for neuroblastoma, resistance, invasiveness, and metastatic mobility limit the treatment efficacy. Therefore, it is necessary to develop new strategies for treating neuroblastoma. The present study aimed to evaluate the anticancer effects of nafamostat mesylate, a previously known serine protease inhibitor, on neuroblastoma cells. Effects of nafamostat mesylate on neuroblastoma cell migration and proliferation were analyzed by wound healing assay and WST‑8 assay, respectively. To elucidate the mechanisms underlying the effects of nafamostat mesylate on neuroblastoma, the expression levels of NF‑κB were measured via western blotting, and the production of the cytokine vascular endothelial growth factor (VEGF) in the cell culture supernatants was determined via ELISA. In addition, a mouse model of hematogenous metastasis was used to investigate the effects of nafamostat mesylate on neuroblastoma. It was determined that nafamostat mesylate significantly inhibited migration and invasion of Neuro‑2a cells, but it had no effect on cell proliferationat 24 h after treatment. Exposure of Neuro‑2a cells to nafamostat mesylate resulted in decreased vascular endothelial growth factor production, which could be a pivotal mechanism underlying the inhibitory effects of neuroblastoma metastasis. The results of the present study suggest that nafamostat mesylate may be an effective treatment against neuroblastoma invasion and metastasis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Pediatrics, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan","subitem_description_type":"Other"},{"subitem_description":"6p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙医学第1081号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3892/mco.2022.2571","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"MOLECULAR AND CLINICAL ONCOLOGY 17: 138, 2022に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MORIMOTO, MARI","creatorNameLang":"en"},{"creatorName":"モリモト, マリ","creatorNameLang":"ja-Kana"},{"creatorName":"森本, 真理","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-04-05"}],"displaytype":"detail","filename":"2022DM1220.pdf","filesize":[{"value":"725.7 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2022DM1220","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/15531/files/2022DM1220.pdf"},"version_id":"241f4c3b-e457-4fa7-8ac7-a5bf258b646c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"nafamostat mesylate","subitem_subject_scheme":"Other"},{"subitem_subject":"neuroblastoma","subitem_subject_scheme":"Other"},{"subitem_subject":"metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"murine model","subitem_subject_scheme":"Other"},{"subitem_subject":"vascular endothelial growth factor","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Nafamostat mesylate prevents metastasis and dissemination of neuroblastoma through vascular endothelial growth factor inhibition","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nafamostat mesylate prevents metastasis and dissemination of neuroblastoma through vascular endothelial growth factor inhibition","subitem_title_language":"en"}]},"item_type_id":"7","owner":"16","path":["1336"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-04-05"},"publish_date":"2023-04-05","publish_status":"0","recid":"15531","relation_version_is_last":true,"title":["Nafamostat mesylate prevents metastasis and dissemination of neuroblastoma through vascular endothelial growth factor inhibition"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2023-10-17T01:54:41.953416+00:00"}