{"created":"2023-08-18T04:32:13.373955+00:00","id":2000013,"links":{},"metadata":{"_buckets":{"deposit":"69df53d6-9a09-4faf-9dbe-db7b49850aa8"},"_deposit":{"created_by":15,"id":"2000013","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000013"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000013","sets":["420:684:685"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-10-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"65","bibliographicPageStart":"60","bibliographicVolumeNumber":"625","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glycoside hydrolase family 94 (GH94) contains enzymes that reversibly catalyze the phosphorolysis of β-glycosides. We conducted this study to investigate a GH94 protein (PBOR_13355) encoded in the genome of Paenibacillus borealis DSM 13188 with low sequence identity to known phosphorylases. Screening of acceptor substrates for reverse phosphorolysis in the presence of α-D-glucose 1-phosphate as a donor substrate showed that PBOR_13355 utilized D-glucuronic acid and p-nitrophenyl β-D-glucuronide as acceptors. In the reaction with D-glucuronic acid, 3-O-β-D-glucopyranosyl-D-glucuronic acid was synthesized. PBOR_13355 showed a higher apparent catalytic efficiency to p-nitrophenyl β-D-glucuronide than to D-glucuronic acid, and thus, PBOR_13355 was concluded to be a novel glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase. PBOR_13360, encoded by the gene immediately downstream of the PBOR_13355 gene, was shown to be β-glucuronidase. Collectively, PBOR_13355 and PBOR_13360 are predicted to work together in the cytosol to metabolize oligosaccharides containing the 3-O-β-D-glucopyranosyl-β-D-glucuronide structure released from bacterial and plant acidic carbohydrates.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2022.07.098","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1090-2104","subitem_source_identifier_type":"PISSN"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Isono,Naoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mizutani,Emi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hayashida,Haruka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Katsuzaki,Hirotaka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saburi,Wataru","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-08-18"}],"filename":"2023JA0004.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://mie-u.repo.nii.ac.jp/record/2000013/files/2023JA0004.pdf"},"version_id":"5a37675e-b9f0-4069-833a-59a6b179e889"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"15","path":["685"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-08-18"},"publish_date":"2023-08-18","publish_status":"0","recid":"2000013","relation_version_is_last":true,"title":["Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-17T02:16:41.712249+00:00"}