{"created":"2023-12-13T01:10:05.965873+00:00","id":2000185,"links":{},"metadata":{"_buckets":{"deposit":"3ea964f1-b780-41a7-9ab5-ca9e8608b20f"},"_deposit":{"created_by":15,"id":"2000185","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000185"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000185","sets":["334:627:1701929182893"]},"author_link":[],"control_number":"2000185","item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-15","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"新たながん免疫療法として急速に進展するCAR-T細胞療法の代謝特性を理解することにより、有効性の向上につなげる目的で本研究を行った。GITRを細胞内ドメインに持つzG CAR-T細胞は28z CAR-T細胞や4-1BBz CAR-T細胞に比べ解糖への依存が少なく、脂肪酸β酸化およびミトコンドリアでの酸化的リン酸化に強く依存している。この代謝特性が高い標的細胞傷害活性と関連する可能性が示唆された。","subitem_description_type":"Other"},{"subitem_description":"We aimed to improve the efficacy of CAR-T cell therapy by understanding the energy metabolism of CAR-T cells in this study. We demonstrated that zG CAR-T cell, comprising GITR (Glucocorticoid-induced TNF-related Protein) in cytoplasmic domain had less dependency on glycolysis, and relied more on fatty acid beta oxidation and oxidative phosphorylation in mitochondria than CAR-T cells having 28z or 4-1BB. Real-time cytotoxicity assay revealed that zG CD19 CAR-T cells killed target leukemia cell line (NALM6) more efficiently than 28z or 4-1BBz CD19 CAR-T cells (gammadelta CAR-T). Superior target-specific cytotoxicity of zG CAR-T cells might be associated with OXPHOS-dominant metabolic characteristics.","subitem_description_type":"Other"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2021年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K07751","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三輪, 啓志","creatorNameLang":"ja"},{"creatorName":"MIWA, Hiroshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-13"}],"filename":"2023RP0021.pdf","filesize":[{"value":"212 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://mie-u.repo.nii.ac.jp/record/2000185/files/2023RP0021.pdf"},"version_id":"f9e0e51a-e97c-4c6b-92d6-fdda6bb6b3d0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CAR-T細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"エネルギー代謝","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"腫瘍細胞とキメラ抗原受容体Ｔ細胞のエネルギー代謝の相違に着目した次世代細胞治療","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"腫瘍細胞とキメラ抗原受容体Ｔ細胞のエネルギー代謝の相違に着目した次世代細胞治療","subitem_title_language":"ja"},{"subitem_title":"Next generation CAR-T cell therapy considering metabolism of tumor cells and CAR-T cells","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1701929182893"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-12-13"},"publish_date":"2023-12-13","publish_status":"0","recid":"2000185","relation_version_is_last":true,"title":["腫瘍細胞とキメラ抗原受容体Ｔ細胞のエネルギー代謝の相違に着目した次世代細胞治療"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-09-20T01:26:15.921722+00:00"}