{"created":"2023-12-13T04:19:15.136271+00:00","id":2000193,"links":{},"metadata":{"_buckets":{"deposit":"56c82261-267d-4271-80e1-406ff3771cd5"},"_deposit":{"created_by":15,"id":"2000193","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000193"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000193","sets":["334:627:1701929182893"]},"author_link":[],"control_number":"2000193","item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-17","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"進行性前立腺がんに対するホルモン療法を開始する前に、ホルモン療法が有効な患者か、それとも抗がん剤治療を早期に始めるべきか、と判断する指標を、がん細胞周囲に存在する多様な線維芽細胞から見出すことを試みた。今回、我々が同定した線維芽細胞が産生・分泌するエクソソーム内包miR-3121-3pは、前立腺がん細胞におけるがん抑制遺伝子NKX3-1遺伝子発現を増加させる鍵分子の１つである可能性を示唆した。つまり、前立腺がん微小環境には前立腺がん細胞分化を維持するよう働く線維芽細胞が存在していることから、ホルモン療法や抗がん剤治療によってがん抑制的に働く線維芽細胞が傷害されないよう守る必要がある。","subitem_description_type":"Abstract"},{"subitem_description":"The tumor stroma of prostate cancer (PCa) contains functionally different populations of cancer-associated fibroblasts (CAFs). We hypothesize that cancer progression may be predicted by characterizing the CAFs. We investigated the effects of normal PrSC and 3 PCa patient-derived CAFs on cancer-related gene expressions in 4 human PCa sublines differing in androgen receptor (AR) dependency. The mRNA expression of tumor suppressor gene NKX3-1 in PCa cells having AR dependency was significantly increased by treatment with conditioned medium of PrSC and M5. Microarray profiling of exosomal miRNAs derived from PrSC and M5 identified miR- 449c-3p and miR-3121-3p for miRNAs related to NKX3-1 mRNA. The mRNA expression of NKX3-1 in PCa cells having AR dependency was increased by transfection of miRNA mimic (has-miR-3121-3p). These results suggest that fibroblast-derived miR-3121-3p may be a candidate of stromal morphogen for maintaining PCa cell differentiation in PCa cells having AR dependency.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2021年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K09686","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 健一朗","creatorNameLang":"ja"},{"creatorName":"Ishii, Kenichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"佐々木, 豪","creatorNameLang":"ja"},{"creatorName":"Sasaki, Takeshi","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"広川, 佳史","creatorNameLang":"ja"},{"creatorName":"Hirokawa, Yoshifumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"渡邉, 昌俊","creatorNameLang":"ja"},{"creatorName":"Watanabe, Masatoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-13"}],"filename":"2023RP0030.pdf","filesize":[{"value":"94 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://mie-u.repo.nii.ac.jp/record/2000193/files/2023RP0030.pdf"},"version_id":"fc54fb0d-7a5c-4a72-9d70-cb7a2a549da0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"進行性前立腺がん","subitem_subject_scheme":"Other"},{"subitem_subject":"アンドロゲン感受性","subitem_subject_scheme":"Other"},{"subitem_subject":"がん細胞分化","subitem_subject_scheme":"Other"},{"subitem_subject":"がん抑制遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"NKX3-1","subitem_subject_scheme":"Other"},{"subitem_subject":"がん関連線維芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"エクソソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロRNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"がん促進性線維芽細胞由来エクソソームを標的とした前立腺がん新規診断法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"がん促進性線維芽細胞由来エクソソームを標的とした前立腺がん新規診断法の開発","subitem_title_language":"ja"},{"subitem_title":"Development of a novel diagnostic method for prostate cancer that targets tumor promoting fibroblast-derived exosomes","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1701929182893"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-12-13"},"publish_date":"2023-12-13","publish_status":"0","recid":"2000193","relation_version_is_last":true,"title":["がん促進性線維芽細胞由来エクソソームを標的とした前立腺がん新規診断法の開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-09-20T02:21:20.486245+00:00"}