{"created":"2023-12-22T00:13:08.522070+00:00","id":2000267,"links":{},"metadata":{"_buckets":{"deposit":"51be6233-b8ce-4c5c-b441-30a77d90a762"},"_deposit":{"created_by":15,"id":"2000267","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000267"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000267","sets":["420:724:1701929361491"]},"author_link":[],"control_number":"2000267","item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-20","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"3コピーのhLGR5遺伝子を搭載したタンデムリピート型プラスミドをゼブラフィッシュ受精卵にマイクロインジェクションすることで、hLGR5タンパク質を発現することに成功した（特許出願１件）。\n次に、キンギョIg遺伝子についてコイ科およびニジマスのゲノム情報と比較した結果、キンギョゲノムにはIgM遺伝子に加えて、IgZ、IgZ2、IgZ3遺伝子の存在が確認された。さらに、大腸菌曝露前後におけるキンギョIg遺伝子発現量の変動を確認した結果、キンギョ（和金）ではIgZ、IgZ2は鰓でのみ遺伝子発現量が増加し、IgM遺伝子はゼブラフィッシュほどの発現増加は検出されなかった。","subitem_description_type":"Abstract"},{"subitem_description":"A tandem repeat of plasmid carrying three hLGR5 genes was injected into zebrafish fertilized eggs. As a result, Expression of the recombinant hLGR5 in zebrafish embryos was succeeded. (Patent application #1)\nNext, the immunoglobulin genes in goldfish genome were investigated in comparison with the genomic information on zebrafish, carp, and rainbow trout. As a result, the genes encoding IgZ, IgZ2, and IgZ3 in goldfish genome was found in addition to IgM. Furthermore, the gene expression pattern among those Igs was observed before or after exposing Escherichia coli. As a result, although expression of IgZ and IgZ2 in Wakin type goldfish was increased, expression pattern of IgM in Wakin type goldfish was not similar to that of zebrafish IgM.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2021年度科学研究費補助金(基盤研究(B))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19H02524","subitem_description_type":"TechnicalInfo"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田丸, 浩","creatorNameLang":"ja"},{"creatorName":"Tamaru, Yutaka","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-22"}],"filename":"2023RP0087.pdf","filesize":[{"value":"82 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://mie-u.repo.nii.ac.jp/record/2000267/files/2023RP0087.pdf"},"version_id":"fda650e5-4d2a-4da0-a985-6267b33b4753"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"一本鎖抗体","subitem_subject_scheme":"Other"},{"subitem_subject":"キンギョ","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼブラフィッシュ","subitem_subject_scheme":"Other"},{"subitem_subject":"ファージディスプレイ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"魚類免疫系を活用した一本鎖抗体取得システムの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"魚類免疫系を活用した一本鎖抗体取得システムの開発","subitem_title_language":"ja"},{"subitem_title":"Development of the system for obtaining a single-chain antibody using goldfish immunity","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1701929361491"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-12-22"},"publish_date":"2023-12-22","publish_status":"0","recid":"2000267","relation_version_is_last":true,"title":["魚類免疫系を活用した一本鎖抗体取得システムの開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-09-24T06:01:37.806814+00:00"}