{"created":"2024-05-10T02:08:28.889742+00:00","id":2000760,"links":{},"metadata":{"_buckets":{"deposit":"cfdd33b3-7cef-401a-a0fa-e8af074229d5"},"_deposit":{"created_by":15,"id":"2000760","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000760"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000760","sets":["334:608:618:1694066935734"]},"author_link":[],"control_number":"2000760","item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The sexual phase of Plasmodium represents a crucial step in malaria transmission, during which these parasites fertilize and form ookinetes to infect mosquitoes. Plasmodium development after fertilization is thought to proceed with female-stored mRNAs until the formation of a retort-form ookinete; thus, transcriptional activity in zygotes has previously been considered quiescent. In this study, we reveal the essential role of transcriptional activity in zygotes by investigating the function of a newly identified AP2 transcription factor, AP2-Z, in P. berghei.ap2-z was previously reported as a female transcriptional regulator gene whose disruption resulted in developmental arrest at the retort stage of ookinetes. In this study, although ap2-z was transcribed in females, we show that it was translationally repressed by the DOZI complex and translated after fertilization with peak expression at the zygote stage. ChIP-seq analysis of AP2-Z shows that it binds on specific DNA motifs, targeting the majority of genes known as an essential component of ookinetes, which largely overlap with theAP2-O targets, as well as genes that are unique among the targets of other sexual transcription factors. The results of this study also indicate the existence of a cascade of transcription factors, beginning with AP2-G, that proceeds from gametocytogenesis to ookinete formation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Laboratory of Medical Zoology, Department of Medicine, Mie University","subitem_description_type":"Other"},{"subitem_description":"24p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2233号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal. ppat.1010510","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"PLoS Pathogens 2022; 18(8)に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西, 翔","creatorNameLang":"ja"},{"creatorName":"ニシ, ツバサ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishi, Tsubasa","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-05-10"}],"filename":"2023DM0302.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2023DM0302.pdf","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/2000760/files/2023DM0302.pdf"},"version_id":"3eefecb2-79fa-45ca-909b-fdf175f5d617"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development","subitem_title_language":"en"}]},"item_type_id":"7","owner":"15","path":["1694066935734"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-05-10"},"publish_date":"2024-05-10","publish_status":"0","recid":"2000760","relation_version_is_last":true,"title":["Identification of a novel AP2 transcription factor in zygotes with an essential role in Plasmodium ookinete development"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-05-10T02:42:40.612236+00:00"}