@phdthesis{oai:mie-u.repo.nii.ac.jp:02000776,
 author = {岩崎, 竜也 and Iwasaki, Tatsuya},
 month = {Mar},
 note = {application/pdf, Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) contribute to the sensitization of primary afferents and are involved in the pathogenesis of inflammatory pain. The purpose of this preliminary study was to examine the expression of other GFLs (neurturin [NRTN], artemin [ARTN], persephin [PSPN]) and receptors in human IVD cells and tissues exhibiting early and advanced stages of degeneration. Human IVD cells were cultured as a monolayer after isolation from the nucleus pulposus (NP) and anulus fibrosus (AF) tissues. The mRNA expression of NRTN, ARTN, PSPN, and their receptors (GFRA2–GFRA4) was quantified using real-time PCR. Protein expression was evaluated by immunohistochemistry and Western blotting. The expression of NRTN, ARTN, PSPN, and their co- receptors (GFRA2-GFRA4) was identified in human IVD cells at both mRNA and protein levels. A trend was noted wherein the mRNA expression of ARTN, PSPN, and GFRA2 was upregulated by IL-1β treatment in a dose-dependent manner. The percentages of immunopositive cells in the advanced degenerate stage of ARTN, PSPN, and GFRA2 were significantly higher than those in the early degenerate stage. Their expression was enhanced in advanced tissue degeneration, which suggests that GFLs (ARTN and PSPN) may be involved in the pathogenesis of discogenic pain., 本文/Department of Orthopedic Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu City, Mie, Japan, 17p},
 school = {三重大学},
 title = {Expression of Glial Cell Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs},
 year = {2024},
 yomi = {イワサキ, タツヤ}
}