{"created":"2024-07-29T04:37:30.116734+00:00","id":2000840,"links":{},"metadata":{"_buckets":{"deposit":"e576bda0-cc48-4142-8c1c-65ea1b4ff7d0"},"_deposit":{"created_by":15,"id":"2000840","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000840"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000840","sets":["334:608:618:1721969789237"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-07-17","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-07-17"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The eight flavonoids, apigenin, chrysin, hesperidin, kaempferol, myricetin, quercetin, rutin and　luteolin were tested for the inhibition of human parainfluenza virus type 2 (hPIV-2) replication.　Three flavonoids out of the eight, kaempferol, quercetin and luteolin inhibited hPIV-2 replication.　Kaempferol reduced the virus release (below 1/10,000), partly inhibited genome and mRNA　syntheses, but protein synthesis was observed. It partly inhibited virus entry into the cells and virus　spreading, and also partly disrupted microtubules and actin microfilaments, indicating that the virus　release inhibition was partly caused by the disruption of cytoskeleton. Quercetine reduced the virus　release (below 1/10,000), partly inhibited genome, mRNA and protein syntheses. It partly inhibited　virus entry and spreading, and also partly destroyed microtubules and microfilaments. Luteolin　reduced the virus release (below 1/100,000), largely inhibited genome, mRNA and protein　syntheses. It inhibited virus entry and spreading. It disrupted microtubules and microfilaments.　These results indicated that luteolin has the most inhibitory effect on hPIV-2 relication. In　conclusion, the three flavonoids inhibited virus replication by the inhibition of genome, mRNA and　protein syntheses, and in addition to those, by the disruption of cytoskeleton in vitro.","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文/Department of Microbiology, Mie University Graduate School of Medicine, Mie, Japan","subitem_description_type":"Other"},{"subitem_description":"8p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2271号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.5582/ddt.2023.01099","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Drug Discoveries & Therapeutics. 2024; 18(1):16-23に掲載"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"杉野, 香江","creatorNameLang":"ja"},{"creatorName":"スギノ, カエ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakai-Sugino, Kae","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-07-29"}],"filename":"2024DM0706.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","url":{"label":"2024DM0706.pdf","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/2000840/files/2024DM0706.pdf"},"version_id":"5fd511d5-703c-43bb-993c-ef3ed1407df0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Virus replication","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"flavonoid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant green fluorescence protein-expressing hPIV-2 without matrix protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Inhibitory effects of kaempferol, quercetin and luteolin on the replication of human parainfluenza virus type 2 in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibitory effects of kaempferol, quercetin and luteolin on the replication of human parainfluenza virus type 2 in vitro","subitem_title_language":"en"}]},"item_type_id":"7","owner":"15","path":["1721969789237"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-29"},"publish_date":"2024-07-29","publish_status":"0","recid":"2000840","relation_version_is_last":true,"title":["Inhibitory effects of kaempferol, quercetin and luteolin on the replication of human parainfluenza virus type 2 in vitro"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-07-29T05:06:56.761481+00:00"}