{"created":"2024-10-17T02:24:55.493002+00:00","id":2000934,"links":{},"metadata":{"_buckets":{"deposit":"9033080e-a5b7-4971-98ba-30ac745e56cc"},"_deposit":{"created_by":15,"id":"2000934","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000934"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000934","sets":["334:627:1728958044917"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-02","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"エクソソームドラッグデリバリーシステムを利用した後天的トリソミックレスキュー法を構築することを目標に実験を行った。しかし、エクソソームの大量精製に時間を要し、エクソソームデリバリーシステムの確立までは至っていない。\n21番染色体消去プラスミドの改良を重ねた結果、長腕側テロメア付近の13ヵ所のgRNAを連結したCRISPR-Cas9プラスミドで最も効率が高かった（約17%）。さらにdCasに載せ替えたプラスミドを作製したところ、染色体除去率は約4％と低下した。dCasを使用して染色体除去を惹起するためには更なる改良が必要と考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Experiments were conducted with the goal of establishing an acquired trisomic rescue method using an exosomal drug delivery system. However, it took time to purify a large number of exosomes, and we have not yet established an exosome delivery system. \nAfter repeated improvements of chromosome 21 elimination plasmids, the CRISPR-Cas9 plasmid linked with gRNAs at 13 sites near the long arm telomere showed the highest efficiency (about 17%). Further improvement is needed to induce chromosome removal using dCas.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2020年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"20K06758","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"脇田, 幸子","creatorNameLang":"ja"},{"creatorName":"WAKITA, SACHIKO","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"原, 万里","creatorNameLang":"ja"},{"creatorName":"HARA, MARI","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-17"}],"filename":"2024RP0025.pdf","filesize":[{"value":"473 KB"}],"format":"application/pdf","url":{"label":"2024RP0025.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000934/files/2024RP0025.pdf"},"version_id":"0ad6bec3-3e7f-43c4-bd77-7c695dca36e0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エクソソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子編集","subitem_subject_scheme":"Other"},{"subitem_subject":"iPS","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"エクソソームを利用した後天的トリソミックレスキュー法の構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エクソソームを利用した後天的トリソミックレスキュー法の構築","subitem_title_language":"ja"},{"subitem_title":"Constructing an Acquired Trisomic Rescue Method Using Exosomes","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958044917"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-17"},"publish_date":"2024-10-17","publish_status":"0","recid":"2000934","relation_version_is_last":true,"title":["エクソソームを利用した後天的トリソミックレスキュー法の構築"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-17T02:36:36.678533+00:00"}